Regulation of scavenger receptor, class B, type I, a high density lipoprotein receptor, in liver and steroidogenic tissues of the rat

Katherine T. Landschulz, Ravindra K. Pathak, Attilio Rigotti, Monty Krieger, Helen H. Hobbs

Research output: Contribution to journalArticle

434 Citations (Scopus)

Abstract

The scavenger receptor, class B, type I (SR-BI) binds HDL, and mediates the selective transfer of cholesteryl esters from HDL, to cultured cells. The tissue distribution of SR-BI in mice suggests that this receptor may deliver HDL-cholesterol to the liver and to nonplacental steroidogenic tissues. To examine the role of SR-BI in vivo, we determined its tissue and cell type- specific expression pattern and regulation in rats. High levels of immunnodetectable SR-BI were present in the adrenal gland, ovary, and liver. In pregnant animals, the mammary gland also expressed high levels of the protein. SR-BI was localized by immunofluorescence to the surfaces of steroidogenic cells in the zona fasciculata and zona reticularis of the adrenal gland and to the corpus luteal cells of the ovary. High-dose estrogen treatment dramatically reduced SR-BI in the liver and increased SR-BI in the adrenal gland and corpus luteal cells of the ovary. These estrogen-induced increases in SR-BI in the adrenal gland and ovary were accompanied by enhanced in vivo uptake of fluorescent lipid from HDL. The administration of human chorionic gonadotropin induced a dramatic increase in SR-BI in the steroidogenic Leydig cells of the testes. These findings suggest that SR-BI mediates physiologically relevant uptake of cholesterol from HDL to nonplacental steroidogenic tissues in vivo.

Original languageEnglish (US)
Pages (from-to)984-995
Number of pages12
JournalJournal of Clinical Investigation
Volume98
Issue number4
StatePublished - Aug 15 1996

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CD36 Antigens
Liver
Adrenal Glands
Ovary
Luteal Cells
HDL Cholesterol
Estrogens
Zona Reticularis
high density lipoprotein receptors
Zona Fasciculata
Animal Mammary Glands
Leydig Cells
Tissue Distribution
Chorionic Gonadotropin
Fluorescent Antibody Technique
Testis
Cultured Cells

Keywords

  • estrogen
  • high density lipoprotein
  • human chorionic gonadotropin
  • rats
  • receptor

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Regulation of scavenger receptor, class B, type I, a high density lipoprotein receptor, in liver and steroidogenic tissues of the rat. / Landschulz, Katherine T.; Pathak, Ravindra K.; Rigotti, Attilio; Krieger, Monty; Hobbs, Helen H.

In: Journal of Clinical Investigation, Vol. 98, No. 4, 15.08.1996, p. 984-995.

Research output: Contribution to journalArticle

Landschulz, Katherine T. ; Pathak, Ravindra K. ; Rigotti, Attilio ; Krieger, Monty ; Hobbs, Helen H. / Regulation of scavenger receptor, class B, type I, a high density lipoprotein receptor, in liver and steroidogenic tissues of the rat. In: Journal of Clinical Investigation. 1996 ; Vol. 98, No. 4. pp. 984-995.
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