Regulation of superoxide dismutase synthesis in Candida albicans

Uma Gunasekaran; Ruipang Yang; Muthukumaran Gunasekaran

doi:10.1023/A:1006985710874

Regulation of superoxide dismutase synthesis in Candida albicans

Uma Gunasekaran, Ruipang Yang, Muthukumaran Gunasekaran

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The synthesis of superoxide dismutase [SOD: EC 1.15.1.1] in response to various cultural conditions was examined in Candida albicans, an opportunistic yeast which causes candidiasis in immunosuppressed patients. SOD plays an important role in protecting cells from the oxidative damage of superoxide radicals. Maximum SOD activity was found after 72 hrs of yeast growth. The optimum pH and temperature for the SOD activity were 7 and 40 °C, respectively. The major SOD activity was found in the cytosol fraction and the level of extracellular SOD was very low. The enzyme was stimulated to varying degrees by cholic acid, procaine and tocopherol. On the basis of inhibitor studies and other enzyme properties, the isolated enzyme from C. albicans is identified as copper and zinc superoxide dismutase.

Original language	English (US)
Pages (from-to)	59-63
Number of pages	5
Journal	Mycopathologia
Volume	141
Issue number	2
DOIs	https://doi.org/10.1023/A:1006985710874
State	Published - 1998

Keywords

(SOD)
Candida albicans
Superoxide dismutase
Yeast

ASJC Scopus subject areas

Microbiology
Applied Microbiology and Biotechnology
Agronomy and Crop Science
veterinary (miscalleneous)

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10.1023/A:1006985710874

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title = "Regulation of superoxide dismutase synthesis in Candida albicans",

abstract = "The synthesis of superoxide dismutase [SOD: EC 1.15.1.1] in response to various cultural conditions was examined in Candida albicans, an opportunistic yeast which causes candidiasis in immunosuppressed patients. SOD plays an important role in protecting cells from the oxidative damage of superoxide radicals. Maximum SOD activity was found after 72 hrs of yeast growth. The optimum pH and temperature for the SOD activity were 7 and 40 °C, respectively. The major SOD activity was found in the cytosol fraction and the level of extracellular SOD was very low. The enzyme was stimulated to varying degrees by cholic acid, procaine and tocopherol. On the basis of inhibitor studies and other enzyme properties, the isolated enzyme from C. albicans is identified as copper and zinc superoxide dismutase.",

keywords = "(SOD), Candida albicans, Superoxide dismutase, Yeast",

author = "Uma Gunasekaran and Ruipang Yang and Muthukumaran Gunasekaran",

note = "Funding Information: This study is supported in part by the National Science Foundation grant HRD 92-53037, the Kellogg Foundation Grant P0010125, by the Howard Hughes Medical Institute grant 71194-527802 and by the Department of Education grant P-120A30013.",

year = "1998",

doi = "10.1023/A:1006985710874",

language = "English (US)",

volume = "141",

pages = "59--63",

journal = "Mycopathologia",

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T1 - Regulation of superoxide dismutase synthesis in Candida albicans

AU - Gunasekaran, Uma

AU - Yang, Ruipang

AU - Gunasekaran, Muthukumaran

N1 - Funding Information: This study is supported in part by the National Science Foundation grant HRD 92-53037, the Kellogg Foundation Grant P0010125, by the Howard Hughes Medical Institute grant 71194-527802 and by the Department of Education grant P-120A30013.

PY - 1998

Y1 - 1998

N2 - The synthesis of superoxide dismutase [SOD: EC 1.15.1.1] in response to various cultural conditions was examined in Candida albicans, an opportunistic yeast which causes candidiasis in immunosuppressed patients. SOD plays an important role in protecting cells from the oxidative damage of superoxide radicals. Maximum SOD activity was found after 72 hrs of yeast growth. The optimum pH and temperature for the SOD activity were 7 and 40 °C, respectively. The major SOD activity was found in the cytosol fraction and the level of extracellular SOD was very low. The enzyme was stimulated to varying degrees by cholic acid, procaine and tocopherol. On the basis of inhibitor studies and other enzyme properties, the isolated enzyme from C. albicans is identified as copper and zinc superoxide dismutase.

AB - The synthesis of superoxide dismutase [SOD: EC 1.15.1.1] in response to various cultural conditions was examined in Candida albicans, an opportunistic yeast which causes candidiasis in immunosuppressed patients. SOD plays an important role in protecting cells from the oxidative damage of superoxide radicals. Maximum SOD activity was found after 72 hrs of yeast growth. The optimum pH and temperature for the SOD activity were 7 and 40 °C, respectively. The major SOD activity was found in the cytosol fraction and the level of extracellular SOD was very low. The enzyme was stimulated to varying degrees by cholic acid, procaine and tocopherol. On the basis of inhibitor studies and other enzyme properties, the isolated enzyme from C. albicans is identified as copper and zinc superoxide dismutase.

KW - (SOD)

KW - Candida albicans

KW - Superoxide dismutase

KW - Yeast

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SN - 0301-486X

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JO - Mycopathologia

JF - Mycopathologia

IS - 2

ER -

Regulation of superoxide dismutase synthesis in Candida albicans

Abstract

Keywords

ASJC Scopus subject areas

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