Regulation of T-cell receptor γ-chain RNA expression in murine Thy-1+ dendritic epidermal cells

William A. Kuziel, Akira Takashima, Mark Bonyhadi, Paul R. Bergstresser, James P. Allison, Robert E. Tigelaar, Philip W. Tucker

Research output: Contribution to journalArticle

152 Scopus citations

Abstract

The epidermis of normal mice contains two distinct populations of dendritic cells derived from the bone marrow, Ia+ Langerhans cells and Ia - cells that express the Thy-1 alloantigen1-5. The Thy-1-bearing dendritic epidermal cells (Thy-1+ dEC) have a surface phenotype similar to that of very early T-lineage cells6-8, produce IL-2-like growth factors10 and exhibit cytotoxicity which is not restricted by the major histocompatibility complex (MHC)10,11. The relationship of Thy-1+ dEC to the T-cell lineage is unclear. Most T lymphocytes bear a receptor for antigen composed of an α chain and a β chain12-17 associated with a nonpoly-morphic complex termed CD3 (T3)18-21. A minor population carries a receptor in which CD3 is associated with a γ/δ complex21-25. We have analysed clones of Thy-1+ dEC for rearrangement and expression of the genes for the α-, β- and γ-chains of the T-cell receptor (TCR). They do not express α or β but do carry a γ/δ complex. Activation of the cells with Con A is associated with a rapid decrease in the steady-state level of γ-chain RNA. Because Thy-1+ dEC resemble early stage T lymphocytes, down-regulation of TCR expression may reflect a necessary event during T cell differentiation.

Original languageEnglish (US)
Pages (from-to)263-266
Number of pages4
JournalNature
Volume328
Issue number6127
Publication statusPublished - 1988

    Fingerprint

ASJC Scopus subject areas

  • General

Cite this

Kuziel, W. A., Takashima, A., Bonyhadi, M., Bergstresser, P. R., Allison, J. P., Tigelaar, R. E., & Tucker, P. W. (1988). Regulation of T-cell receptor γ-chain RNA expression in murine Thy-1+ dendritic epidermal cells. Nature, 328(6127), 263-266.