Regulation of T-cell receptor γ-chain RNA expression in murine Thy-1+ dendritic epidermal cells

William A. Kuziel, Akira Takashima, Mark Bonyhadi, Paul R. Bergstresser, James P. Allison, Robert E. Tigelaar, Philip W. Tucker

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

The epidermis of normal mice contains two distinct populations of dendritic cells derived from the bone marrow, Ia+ Langerhans cells and Ia - cells that express the Thy-1 alloantigen1-5. The Thy-1-bearing dendritic epidermal cells (Thy-1+ dEC) have a surface phenotype similar to that of very early T-lineage cells6-8, produce IL-2-like growth factors10 and exhibit cytotoxicity which is not restricted by the major histocompatibility complex (MHC)10,11. The relationship of Thy-1+ dEC to the T-cell lineage is unclear. Most T lymphocytes bear a receptor for antigen composed of an α chain and a β chain12-17 associated with a nonpoly-morphic complex termed CD3 (T3)18-21. A minor population carries a receptor in which CD3 is associated with a γ/δ complex21-25. We have analysed clones of Thy-1+ dEC for rearrangement and expression of the genes for the α-, β- and γ-chains of the T-cell receptor (TCR). They do not express α or β but do carry a γ/δ complex. Activation of the cells with Con A is associated with a rapid decrease in the steady-state level of γ-chain RNA. Because Thy-1+ dEC resemble early stage T lymphocytes, down-regulation of TCR expression may reflect a necessary event during T cell differentiation.

Original languageEnglish (US)
Pages (from-to)263-266
Number of pages4
JournalNature
Volume328
Issue number6127
StatePublished - 1988

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Langerhans Cells
T-Cell Antigen Receptor
RNA
T-Lymphocytes
CD3 Antigens
T-Cell Receptor Genes
Antigen Receptors
Cell Lineage
Major Histocompatibility Complex
Epidermis
Dendritic Cells
Population
Interleukin-2
Cell Differentiation
Down-Regulation
Clone Cells
Bone Marrow
Phenotype
Growth

ASJC Scopus subject areas

  • General

Cite this

Kuziel, W. A., Takashima, A., Bonyhadi, M., Bergstresser, P. R., Allison, J. P., Tigelaar, R. E., & Tucker, P. W. (1988). Regulation of T-cell receptor γ-chain RNA expression in murine Thy-1+ dendritic epidermal cells. Nature, 328(6127), 263-266.

Regulation of T-cell receptor γ-chain RNA expression in murine Thy-1+ dendritic epidermal cells. / Kuziel, William A.; Takashima, Akira; Bonyhadi, Mark; Bergstresser, Paul R.; Allison, James P.; Tigelaar, Robert E.; Tucker, Philip W.

In: Nature, Vol. 328, No. 6127, 1988, p. 263-266.

Research output: Contribution to journalArticle

Kuziel, WA, Takashima, A, Bonyhadi, M, Bergstresser, PR, Allison, JP, Tigelaar, RE & Tucker, PW 1988, 'Regulation of T-cell receptor γ-chain RNA expression in murine Thy-1+ dendritic epidermal cells', Nature, vol. 328, no. 6127, pp. 263-266.
Kuziel WA, Takashima A, Bonyhadi M, Bergstresser PR, Allison JP, Tigelaar RE et al. Regulation of T-cell receptor γ-chain RNA expression in murine Thy-1+ dendritic epidermal cells. Nature. 1988;328(6127):263-266.
Kuziel, William A. ; Takashima, Akira ; Bonyhadi, Mark ; Bergstresser, Paul R. ; Allison, James P. ; Tigelaar, Robert E. ; Tucker, Philip W. / Regulation of T-cell receptor γ-chain RNA expression in murine Thy-1+ dendritic epidermal cells. In: Nature. 1988 ; Vol. 328, No. 6127. pp. 263-266.
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