Regulation of telomere length and suppression of genomic instability in human somatic cells by Ku86

Kyungjae Myung, Goutam Ghosh, Farjana J. Fattah, Gang Li, Haeyoung Kim, Amalia Dutia, Evgenia Pak, Stephanie Smith, Eric A. Hendrickson

Research output: Contribution to journalArticle

73 Scopus citations

Abstract

Ku86 plays a key role in nonhomologous end joining in organisms as evolutionarily disparate as bacteria and humans. In eukaryotic cells, Ku86 has also been implicated in the regulation of telomere length although the effect of Ku86 mutations varies considerably between species. Indeed, telomeres either shorten significantly, shorten slightly, remain unchanged, or lengthen significantly in budding yeast, fission yeast, chicken cells, or plants, respectively, that are null for Ku86 expression. Thus, it has been unclear which model system is most relevant for humans. We demonstrate here that the functional inactivation of even a single allele of Ku86 in human somatic cells results in profound telomere loss, which is accompanied by an increase in chromosomal fusions, translocations, and genomic instability. Together, these experiments demonstrate that Ku86, separate from its role in nonhomologous end joining, performs the additional function in human somatic cells of suppressing genomic instability through the regulation of telomere length.

Original languageEnglish (US)
Pages (from-to)5050-5059
Number of pages10
JournalMolecular and cellular biology
Volume24
Issue number11
DOIs
StatePublished - Jun 1 2004
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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