Regulation of the Anaphase-promoting Complex by the Dual Specificity Phosphatase Human Cdc14a

Joshua Bembenek, Hongtao Yu

Research output: Contribution to journalArticle

83 Scopus citations

Abstract

Two forms of the anaphase-promoting complex (APC) mediate the degradation of critical cell cycle regulators. APCCdc20 promotes sister-chromatid separation by ubiquitinating securin, whereas APC Cdh1 ubiquitinates mitotic cyclins, allowing the exit from mitosis. Here we show that phosphorylation of human Cdh1 (hCdh1) by cyclin B-Cdc2 alters the conformation of hCdh1 and prevents it from activating APC. A human homologue of yeast Cdc14, human Cdc14a (hCdc14a), dephosphorylates hCdh1 and activates APCCdh1. In contrast, hCdc14a does not affect the activity of APCCdc20. hCdc14a is a major phosphatase for hCdh1 and localizes to centrosomes in HeLa cells. Therefore, hCdc14a may promote the activation of APCCdh1 and exit from mitosis in mammalian cells.

Original languageEnglish (US)
Pages (from-to)48237-48242
Number of pages6
JournalJournal of Biological Chemistry
Volume276
Issue number51
DOIs
StatePublished - Dec 21 2001

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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