Regulation of the lmo2 promoter during hematopoietic and vascular development in zebrafish

Hao Zhu, David Traver, Alan J. Davidson, Anthony Dibiase, Christine Thisse, Bernard Thisse, Stephen Nimer, Leonard I. Zon

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The Lmo2 transcription factor, a T-cell oncoprotein, is required for both hematopoiesis and angiogenesis. To investigate the fate of lmo2-expressing cells and the transcriptional regulation of lmo2 in vivo, we generated stable transgenic zebrafish that express green fluorescent protein (EGFP) or DsRed under the control of an lmo2 promoter. A 2.5-kb fragment contains the cis-regulatory elements required to recapitulate endogenous lmo2 expression in embryonic hematopoietic and vascular tissues. We further characterized embryonic Lmo2+ cells through transplantation into vlad tepes (vlt), an erythropoietic mutant. These Lmo2+ primitive wave donor cells differentiated into circulating hematopoietic cells and extended the life span of vlt recipients, but did not demonstrate long-term repopulation of the erythroid lineage. Promoter analysis identified a 174-bp proximal promoter that was sufficient to recapitulate lmo2 expression. This element contains critical ETS-binding sites conserved between zebrafish and pufferfish. Furthermore, we show that ets1 is coexpressed with lmo2, and overexpression experiments indicate that ets1 can activate the lmo2 promoter through this element. Our studies elucidate the transcriptional regulation of this key transcription factor, and provide a transgenic system for the functional analysis of blood and blood vessels in zebrafish.

Original languageEnglish (US)
Pages (from-to)256-269
Number of pages14
JournalDevelopmental Biology
Volume281
Issue number2
DOIs
StatePublished - May 15 2005

Fingerprint

Zebrafish
Blood Vessels
Transcription Factors
Tetraodontiformes
Oncogene Proteins
Cell Transplantation
Hematopoiesis
Green Fluorescent Proteins
Binding Sites
T-Lymphocytes

Keywords

  • Hematopoiesis
  • Mesoderm
  • Transgenic
  • Vasculogenesis
  • Zebrafish

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Zhu, H., Traver, D., Davidson, A. J., Dibiase, A., Thisse, C., Thisse, B., ... Zon, L. I. (2005). Regulation of the lmo2 promoter during hematopoietic and vascular development in zebrafish. Developmental Biology, 281(2), 256-269. https://doi.org/10.1016/j.ydbio.2005.01.034

Regulation of the lmo2 promoter during hematopoietic and vascular development in zebrafish. / Zhu, Hao; Traver, David; Davidson, Alan J.; Dibiase, Anthony; Thisse, Christine; Thisse, Bernard; Nimer, Stephen; Zon, Leonard I.

In: Developmental Biology, Vol. 281, No. 2, 15.05.2005, p. 256-269.

Research output: Contribution to journalArticle

Zhu, H, Traver, D, Davidson, AJ, Dibiase, A, Thisse, C, Thisse, B, Nimer, S & Zon, LI 2005, 'Regulation of the lmo2 promoter during hematopoietic and vascular development in zebrafish', Developmental Biology, vol. 281, no. 2, pp. 256-269. https://doi.org/10.1016/j.ydbio.2005.01.034
Zhu, Hao ; Traver, David ; Davidson, Alan J. ; Dibiase, Anthony ; Thisse, Christine ; Thisse, Bernard ; Nimer, Stephen ; Zon, Leonard I. / Regulation of the lmo2 promoter during hematopoietic and vascular development in zebrafish. In: Developmental Biology. 2005 ; Vol. 281, No. 2. pp. 256-269.
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