Regulation of the M1 muscarinic receptor-Gg-phospholipase C-β pathway by nucleotide exchange and GTP hydrolysis

Elliott M. Ross, Gabriel Berstein

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

M1 muscarinic choligernic receptor, Gq and G11 (G q 11), and phospholipase C-β1 were highly purified from both natural sources and cells that express the appropriate cDNA's. When the proteins were co-reconstituted in into phospholipid vesicles, the receptor efficiently and selectively promoted the activation of if G q 11, leading to marked stimulation of PLC activity in the presence of GTPγS. No stimulation was observed in the presence of GTP, however, which led to the finding that PLC-β1 stimulates the hydrolysis of if G q 11-bound GTP at leasr 50-fold. Thus, PLC-β1 is a GTPase activating protein, a GAP, for its physiologic regulator G in q 11. We discuss the implications of PLC- β1's GAP activity on the M1 muscarinic cholinergic signaling pathway.

Original languageEnglish (US)
Pages (from-to)413-419
Number of pages7
JournalLife Sciences
Volume52
Issue number5-6
DOIs
StatePublished - 1993

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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