Regulation of the murine Dδ2 promoter by upstream stimulatory factor 1, Runx1, and c-Myb

Accessibility control of V(D)J recombination at Ag receptor loci depends on the coordinate activities of transcriptional enhancers and germline promoters. Recombination of murine Tcrd gene segments is known to be regulated, at least in part, by the Tcrd enhancer (Eδ) situated in the Jδ2-Cδ intron. However, there has been little characterization of promoters and other cis-acting elements that are activated by or collaborate with Eδ and that might function to regulate Tcrd gene recombination events. We now describe a strong promoter that is tightly associated with the murine Dδ2 gene segment. EMSAs reveal that upstream stimulatory factor 1, Runx1, c-Myb, lymphoid enhancer binding factor 1, NF1, aad E47 all interact with this promoter in vitro. Of these, upstream stimulatory factor 1, Runx1, and c-Myb appear necessary for full promoter activity in transiently transfected cells. Moreover, the same three factors were found to interact with the promoter in vivo by chromatin immunoprecipitative. We suggest that these factors play important roles as Dδ-dependent regulators of δ2 accessibility in vivo. Consistent with the established roles of c-Myb and Runx factors in Eδ function, we detected low level, enhancer-independent activity of the Dδ2 promoter in transient transfection experiments. We speculate that the Dδ2 promoter may play a role as a weak, enhancer-independent regulator in vivo, and might contribute to residual Tcrd rearrangement in Eδ^-/- mice.