Regulation of the Na+/K+ ATPase by Klotho

Mentor Sopjani, Ioana Alesutan, Miribane Dërmaku-Sopjani, Shuchen Gu, Christine Zelenak, Carlos Munoz, Ana Velic, Michael Föller, Kevin P. Rosenblatt, Makoto Kuro-O, Florian Lang

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Klotho-hypomorphic (Klothohm) mice suffer from renal salt wasting and hypovolemia despite hyperaldosteronism. The present study explored the effect of Klotho on renal Na+/K+ ATPase activity. According to immunohistochemistry and confocal microscopy Na+/K + ATPase protein abundance in isolated collecting ducts was lower in Klothohm mice than in their wild type littermates (Klotho +/+). Analysis with dual electrode voltage clamp recording showed that expression of Klotho in Xenopus oocytes increased the Na+/K + ATPase pump current. Treatment of Xenopus oocytes with Klotho protein similarly increased the pump current. In conclusion, Klotho increases the membrane abundance and activity of the Na+/K+ ATPase. Decreased Na+/K+ ATPase activity could thus contribute to the volume-depletion of klothohm mice.

Original languageEnglish (US)
Pages (from-to)1759-1764
Number of pages6
JournalFEBS Letters
Volume585
Issue number12
DOIs
StatePublished - Jun 23 2011

Keywords

  • Protein stability
  • Pump activity
  • Salt depletion
  • Sodium/potassium ATPase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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