Regulation of the RAD2 gene of Saccharomyces cervisiae

W. Siede, G. W. Robinson, D. Kalainov, T. Malley, E. C. Friedberg

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Abstract

Regulation of the DNA damage‐inducible RAD2 gene was investigated in yeast cells transformed with centromeric plasmids containing RAD2‐lacZ fusion constructs. Deletion analysis defined several regions in the 350 bp region upstream of the translational start codon which are required for induction of β‐galactosidase activity. No deletions resulted in constitutivety enhanced expression. We therefore conclude that induction of RAD2 by DNA‐damaging agents is positively regulated. Two domains required for induction have a similar sequence and are located ∼70 and ∼140bp upstream of the major transcriptional start site. Four other sequence domains required for induction contain uninterrupted poly(dA) poly(dT) stretches 9‐13 bp long. Deletion of some of these AT‐rich domains also affects constitutive expression of RAD2. Expression of RAD2 is not cell‐cycle‐regulated in mitotic cells. However, meiosis is accompanied by increased steady‐state levels of RAD2 mRNA in the absence of DNA damage. This enhanced transcription is not dependent on the presence of upstream sequences required for regulation of induction by DNA damage. Increased steady‐state levels of RAD2 mRNA are induced by cycloheximide in asynchronously dividing populations of cells, but not in non‐replicating cells arrested in G1 phase of the cell cycle. Following exposure to u.v. irradiation induction is also dramatically reduced in non‐replicating cells.

Original languageEnglish (US)
Pages (from-to)1697-1707
Number of pages11
JournalMolecular Microbiology
Volume3
Issue number12
DOIs
StatePublished - Dec 1989

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ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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