Regulation of the yeast RAD2 gene DNA damage-dependent induction correlates with protein binding to regulatory sequences and their deletion influences survival

Wolfram Siede, Errol C. Friedberg

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

In the yeast Saccharomyces cerevisiae the RAD2 gene is absolutely required for damage-specific incision of DNA during nucleotide excision repair and is inducible by DNA-damaging agents. In the present study we correlated sensitivity to killing by DNA-damaging agents with the deletion of previously defined specific promoter elements. Deletion of the element DRE2 increased the UV sensitivity of cells in both the G1/early S and S/G2 phases of the cell cycle as well as in stationary phase. On the other hand, increased UV sensitivity associated with deletion of the sequence-related element DRE1 was restricted to cells irradiated in G1/S. Specific binding of protein(s) to the promoter elements DRE1 and DRE2 was observed under non-inducing conditions using gel retardation assays. Exposure of cells to DNA-damaging agents resulted in increased protein binding that was dependent on de novo protein synthesis.

Original languageEnglish (US)
Pages (from-to)247-256
Number of pages10
JournalMGG Molecular & General Genetics
Volume232
Issue number2
DOIs
Publication statusPublished - Mar 1992

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Keywords

  • DNA damage
  • Excision repair
  • Gene regulation
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Genetics

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