Regulation of transforming growth factor expression in rat intestinal epithelial cell Lines

S. Suemori, C. Ciacci, D. K. Podolsky

Research output: Contribution to journalArticle

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Abstract

Autocrine and paracrine modulation of transforming growth factor expression was assessed in rat intestinal epithelial cell lines designated IEC-6 and IEC-17. Addition of the transforming growth factor α (TGFα) homologue epidermal growth factor (EGF) to media of subconfluent IEC-6 cells led to autocrine stimulation of TGFα expression as well as increased expression of the transforming growth factor β1 (TGFβ1). Increased expression of TGFα was maximal between 3 and 6 h after addition of EGF and subsequently declined coincident with increasing level of expression of TGFβ1, which achieved maximal levels 6 h after addition of EGF and was sustained for more than 12 h. Addition of TGFβ1, also led to autocrine induction of its own expression coincident with suppression of TGFα expression. Addition of TGFβ1 was associated with increased expression of β-actin when standardized to a constitutive transcript (GAPDH). Similar responses to addition of EGF and TGFβ1 were observed in another intestinal epithelial cell line, designated IEC-17. Modulation of expression of TGFs was attenuated when cells were grown on the complex extracellular matrix produced by the Engelbreth-Holm-Swarm tumor (Matrigel), reflecting the baseline induction of TGFβ1 expression when compared to IEC-6 and IEC-17 cells maintained on plastic. These observations suggest that expression of TGFs is controlled by autocrine mechanisms in intestinal epithelial cell lines and proliferation stimulated by TGFα may be initially self-reinforcing but ultimately downregulated by induction of TGFα1.

Original languageEnglish (US)
Pages (from-to)2216-2221
Number of pages6
JournalJournal of Clinical Investigation
Volume87
Issue number6
StatePublished - Jun 1991

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Transforming Growth Factors
Epithelial Cells
Cell Line
Epidermal Growth Factor
Plastics
Extracellular Matrix
Actins
Down-Regulation
Cell Proliferation

Keywords

  • Autocrine
  • Epidermal growth factor
  • Extracellular matrix
  • Paracrine

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Regulation of transforming growth factor expression in rat intestinal epithelial cell Lines. / Suemori, S.; Ciacci, C.; Podolsky, D. K.

In: Journal of Clinical Investigation, Vol. 87, No. 6, 06.1991, p. 2216-2221.

Research output: Contribution to journalArticle

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