@article{78e19227279b406690d42bd4bfc244a8,
title = "Regulation of translation by methylation multiplicity of 18S rRNA",
abstract = "N6-methyladenosine (m6A) is a conserved ribonucleoside modification that regulates many facets of RNA metabolism. Using quantitative mass spectrometry, we find that the universally conserved tandem adenosines at the 3′ end of 18S rRNA, thought to be constitutively di-methylated (m62A), are also mono-methylated (m6A). Although present at substoichiometric amounts, m6A at these positions increases significantly in response to sulfur starvation in yeast cells and mammalian cell lines. Combining yeast genetics and ribosome profiling, we provide evidence to suggest that m6A-bearing ribosomes carry out translation distinctly from m62A-bearing ribosomes, featuring a striking specificity for sulfur metabolism genes. Our work thus reveals methylation multiplicity as a mechanism to regulate translation.",
author = "Kuanqing Liu and Santos, {Daniel A.} and Hussmann, {Jeffrey A.} and Yun Wang and Sutter, {Benjamin M.} and Weissman, {Jonathan S.} and Tu, {Benjamin P.}",
note = "Funding Information: We thank Dr. David Bedwell from University of Alabama at Birmingham for the generous gifts of pDB722 and pDB868. We also thank Dr. Juan Manuel Povedano for critically reading the manuscript and Venkat Malladi at the Bioinformatics Core Facility of UT Southwestern Medical Center for assistance with sequencing data analysis. J.S.W. is supported by the Howard Hughes Medical Institute . D.A.S. is supported by the National Science Foundation ( 1650113 ) and Moritz-Heyman Discovery Fellowship. J.A.H. is the Rebecca Ridley Kry Fellow of the Damon Runyon Cancer Research Foundation ( DRG-2262-16 ). B.P.T. is supported by the Welch Foundation ( I-1797 ) and NIH (grants R35GM136370 and R01NS115546 ). Publisher Copyright: {\textcopyright} 2021 The Authors",
year = "2021",
month = mar,
day = "9",
doi = "10.1016/j.celrep.2021.108825",
language = "English (US)",
volume = "34",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "10",
}