Regulation of translation by methylation multiplicity of 18S rRNA

Kuanqing Liu, Daniel A. Santos, Jeffrey A. Hussmann, Yun Wang, Benjamin M. Sutter, Jonathan S. Weissman, Benjamin P. Tu

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


N6-methyladenosine (m6A) is a conserved ribonucleoside modification that regulates many facets of RNA metabolism. Using quantitative mass spectrometry, we find that the universally conserved tandem adenosines at the 3′ end of 18S rRNA, thought to be constitutively di-methylated (m62A), are also mono-methylated (m6A). Although present at substoichiometric amounts, m6A at these positions increases significantly in response to sulfur starvation in yeast cells and mammalian cell lines. Combining yeast genetics and ribosome profiling, we provide evidence to suggest that m6A-bearing ribosomes carry out translation distinctly from m62A-bearing ribosomes, featuring a striking specificity for sulfur metabolism genes. Our work thus reveals methylation multiplicity as a mechanism to regulate translation.

Original languageEnglish (US)
Article number108825
JournalCell Reports
Issue number10
StatePublished - Mar 9 2021

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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