Regulation of vasculogenesis and angiogenesis by EphB/ephrin-B2 signaling between endothelial cells and surrounding mesenchymal cells

Yuichi Oike, Yasuhiro Ito, Koichi Hamada, Xiu Qin Zhang, Keishi Miyata, Fumio Arai, Tomohisa Inada, Kimi Araki, Naomi Nakagata, Motohiro Takeya, Yaz Y. Kisanuki, Masashi Yanagisawa, Nicholas W. Gale, Toshio Suda

Research output: Contribution to journalArticle

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Abstract

Although the cellular and molecular mechanisms governing angiogenesis are only beginning to be understood, signaling through endothelial-restricted receptors, particularly receptor tyrosine kinases, has been shown to play a pivotal role in these events. Recent reports show that EphB receptor tyrosine kinases and their transmembrane-type ephrin-B2 ligands play essential roles in the embryonic vasculature. These studies suggest that cell-to-cell repellent effects due to bidirectional EphB/ephrin-B2 signaling may be crucial for vascular development, similar to the mechanism described for neuronal development. To test this hypothesis, we disrupted the precise expression pattern of EphB/ephrin-B2 in vivo by generating transgenic (CAGp-ephrin-B2 Tg) mice that express ephrin-B2 under the control of a ubiquitous and constitutive promoter, CMV enhancer-β-actin promoter-β-globin splicing acceptor (CAG). These mice displayed an abnormal segmental arrangement of intersomitic vessels, while such anomalies were not observed in Tie-2p-ephrin-B2 Tg mice in which ephrin-B2 was overexpressed in only vascular endothelial cells (ECs). This finding suggests that non-ECs expressing ephrin-B2 alter the migration of ECs expressing EphB receptors into the intersomitic region where ephrin-B2 expression is normally absent. CAGpephrin-B2 Tg mice show sudden death at neonatal stages from aortic dissecting aneurysms due to defective recruitment of vascular smooth muscle cells to the ascending aorta. EphB/ephrin-B2 signaling between endothelial cells and surrounding mesenchymal cells plays an essential role in vasculogenesis, angiogenesis, and vessel maturation.

Original languageEnglish (US)
Pages (from-to)1326-1333
Number of pages8
JournalBlood
Volume100
Issue number4
StatePublished - Aug 15 2002

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Ephrin-B2
Endothelial cells
Endothelial Cells
Eph Family Receptors
Cells
Dissecting Aneurysm
Globins
Aortic Aneurysm
Receptor Protein-Tyrosine Kinases
Sudden Death
Vascular Smooth Muscle
Protein-Tyrosine Kinases
Smooth Muscle Myocytes
Blood Vessels
Muscle
Aorta
Actins

ASJC Scopus subject areas

  • Hematology

Cite this

Oike, Y., Ito, Y., Hamada, K., Zhang, X. Q., Miyata, K., Arai, F., ... Suda, T. (2002). Regulation of vasculogenesis and angiogenesis by EphB/ephrin-B2 signaling between endothelial cells and surrounding mesenchymal cells. Blood, 100(4), 1326-1333.

Regulation of vasculogenesis and angiogenesis by EphB/ephrin-B2 signaling between endothelial cells and surrounding mesenchymal cells. / Oike, Yuichi; Ito, Yasuhiro; Hamada, Koichi; Zhang, Xiu Qin; Miyata, Keishi; Arai, Fumio; Inada, Tomohisa; Araki, Kimi; Nakagata, Naomi; Takeya, Motohiro; Kisanuki, Yaz Y.; Yanagisawa, Masashi; Gale, Nicholas W.; Suda, Toshio.

In: Blood, Vol. 100, No. 4, 15.08.2002, p. 1326-1333.

Research output: Contribution to journalArticle

Oike, Y, Ito, Y, Hamada, K, Zhang, XQ, Miyata, K, Arai, F, Inada, T, Araki, K, Nakagata, N, Takeya, M, Kisanuki, YY, Yanagisawa, M, Gale, NW & Suda, T 2002, 'Regulation of vasculogenesis and angiogenesis by EphB/ephrin-B2 signaling between endothelial cells and surrounding mesenchymal cells', Blood, vol. 100, no. 4, pp. 1326-1333.
Oike Y, Ito Y, Hamada K, Zhang XQ, Miyata K, Arai F et al. Regulation of vasculogenesis and angiogenesis by EphB/ephrin-B2 signaling between endothelial cells and surrounding mesenchymal cells. Blood. 2002 Aug 15;100(4):1326-1333.
Oike, Yuichi ; Ito, Yasuhiro ; Hamada, Koichi ; Zhang, Xiu Qin ; Miyata, Keishi ; Arai, Fumio ; Inada, Tomohisa ; Araki, Kimi ; Nakagata, Naomi ; Takeya, Motohiro ; Kisanuki, Yaz Y. ; Yanagisawa, Masashi ; Gale, Nicholas W. ; Suda, Toshio. / Regulation of vasculogenesis and angiogenesis by EphB/ephrin-B2 signaling between endothelial cells and surrounding mesenchymal cells. In: Blood. 2002 ; Vol. 100, No. 4. pp. 1326-1333.
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