Regulator of G protein signaling-1 modulates paraquat-induced oxidative stress and longevity via the insulin like signaling pathway in Caenorhabditis elegans

Mingyu Wu, Xin Kang, Qiang Wang, Chunyu Zhou, Chandra Mohan, Ai Peng

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Insulin or insulin like signaling (IIS) pathway is a crucial pathway in Caenorhabditis elegans associated with mediating longevity, and stress resistance. Regulators of G protein signaling (RGS) also modulate stress resistance and longevity in multiple in vitro and in vivo models. However, the mechanism underlying RGS mediating stress resistance and longevity remains largely unclear. Here we report that rgs-1, an important member of rgs family, is a novel modulator of IIS pathway in C. elegans. We found that the loss of rgs-1 dramatically promoted paraquat resistance in C. elegans. Further genetic analyses demonstrated that rgs-1 acted downstream of daf-2 and upstream of age-1, pdk-1, daf-16. Instead of affecting those IIS-associated genes in transcriptional process, loss of rgs-1 promoted DAF-16′s nucleus translocation and subset genes’ expression in paraquat-induced oxidative status. By this way, rgs-1 mutant worms exhibited lower ROS damage and longer survival time than wild type worms when both exposed to paraquat. Other than paraquat exposure, rgs-1 mutant also promoted lifespan and cadmium resistance relying on daf-16. As rgs is evolutionarily conserved, our findings open a new insight into rgs family and its role in paraquat-induced oxidative stress and longevity in C. elegans or even mammals.

Original languageEnglish (US)
Pages (from-to)97-105
Number of pages9
JournalToxicology Letters
Volume273
DOIs
StatePublished - May 5 2017

Fingerprint

GTP-Binding Protein Regulators
Paraquat
Oxidative stress
Caenorhabditis elegans
Oxidative Stress
Insulin
Mammals
Cadmium
Gene expression
Modulators
Genes
Gene Expression

Keywords

  • Insulin signaling
  • Longevity
  • Oxidative stress
  • Paraquat
  • rgs-1

ASJC Scopus subject areas

  • Toxicology

Cite this

Regulator of G protein signaling-1 modulates paraquat-induced oxidative stress and longevity via the insulin like signaling pathway in Caenorhabditis elegans. / Wu, Mingyu; Kang, Xin; Wang, Qiang; Zhou, Chunyu; Mohan, Chandra; Peng, Ai.

In: Toxicology Letters, Vol. 273, 05.05.2017, p. 97-105.

Research output: Contribution to journalArticle

Wu, Mingyu ; Kang, Xin ; Wang, Qiang ; Zhou, Chunyu ; Mohan, Chandra ; Peng, Ai. / Regulator of G protein signaling-1 modulates paraquat-induced oxidative stress and longevity via the insulin like signaling pathway in Caenorhabditis elegans. In: Toxicology Letters. 2017 ; Vol. 273. pp. 97-105.
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