Regulatory domains of ca2+/calmodulin-dependent protein kinases

Research output: Contribution to journalArticle

Abstract

Skeletal and smooth muscle myosia light chain kinases (SkMLCK and SmMLCK) and Ca2+/calmodulin-dependent protein kinase II (CaMKII) catalytic cores are regulated intrasterically by respective autoinhibitory and calmodulin (CaM) binding sequence. We have investigated the functional properties of these regulatory segments by expressing chimeric catalytic cores containing different regulatory segments by (denoted in [ ]). SkMLCK and SkMLCK[SmMLCK] had similar activities (V max/Km values) towards regulatory light chain from smooth (SmRLC) and skeletal (SmRLC) muscles. and similar CaM activation properties (Kc aM = 1nM). SkMLCK[CaMKII] also had a similar KCM value but had a 7-fold decrease and undeterminable Vmax/Km values for SmRLC and SkRLC, respectivlely, SkMLcK[nNOS] (neurolnal nitric oxide synthase CaM binidng domain) had no activity although it was expressed. In comparison to CaMKII, CaMKII [SkMLCK] and CaMKII had similar high KCam Values (26-50 nM), but Vmax/Km values for SmRLC were 10-20 fold lower. All kinases bound Cam. except for SkMLCK[nNOS], chimeric activities wee Ca2+/Cam-dependent and were convered to Ca2+/CaM-independent activity after trysin digestion. Thus, but erologous regulatory segments affect substrate recognition and kinase activity. Furthermore, the sensitivity to CaM activation is determined primarliy by the respective catalytic cores. not the CaM binding segments for SkMLCK and CaMKII. (supported in part by the Bashour Research Fund and NIH Grants HL26093 and HL 06296).

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number9
StatePublished - 1997

Fingerprint

Calcium-Calmodulin-Dependent Protein Kinase Type 2
Calcium-Calmodulin-Dependent Protein Kinases
calmodulin
Calmodulin
protein kinases
Catalytic Domain
Phosphotransferases
Cams
Muscle
Skeletal Muscle
Chemical activation
phosphotransferases (kinases)
Light
Organized Financing
Nitric Oxide Synthase
skeletal muscle
Smooth Muscle
calcium-calmodulin-dependent protein kinase
Digestion
calcium

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Regulatory domains of ca2+/calmodulin-dependent protein kinases. / Zhi, G.; Abdullah, S.; Stull, J. T.

In: FASEB Journal, Vol. 11, No. 9, 1997.

Research output: Contribution to journalArticle

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