Regulatory interaction between CFTR and the SLC26 transporters

Nikolay Shcheynikov, Shigeru B H Ko, Weizhong Zeng, Young Choi Joo, Michael R. Dorwart, Philip J. Thomas, Shmuel Muallem

Research output: Chapter in Book/Report/Conference proceedingConference contribution

47 Scopus citations

Abstract

Most epithelia that express CFTR secrete fluid rich in HCO3 - and poor in Cl- that is generated by a CFTR-dependent Cl- absorption and HCO3- secretion process that when aberrant leads to human diseases such as cystic fibrosis and congenital chloride diarrhoea. Epithelial Cl- absorption and HCO 3- secretion require expression of CFTR and other Cl - and HCO3- transporters in the luminal membrane of the secreting cells. Recent advances in understanding this critical epithelial function revealed that the luminal Cl- and HCO 3- transporters are members of the SLC26 family. Characterization of several members of the family reveals that all characterized thus far are electrogenic with an isoform specific Cl-/HCO 3- transport stoichiometry. In vivo these transporters exist in a transporting complex with CFTR. The SLC26 transporters and CFTR are recruited to the complex by binding to scaffolds containing PDZ domains. Upon stimulation and PKA-dependent phosphorylation of CFTR R domain, the R domain binds to the SLC26 transporter STAS domain. Interaction of the R and STAS domains results in a marked and mutual activation of CFTR and the SLC26 transporters. The significance of this mode of regulation to epithelial Cl - absorption and HCO3- secretion is obvious.

Original languageEnglish (US)
Title of host publicationEpithelial Anion Transport in Health and Disease
Subtitle of host publicationThe Role of the SLC26 Transporters Family
Pages177-186
Number of pages10
StatePublished - Dec 1 2006

Publication series

NameNovartis Foundation Symposium
Volume273
ISSN (Print)1528-2511

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ASJC Scopus subject areas

  • Medicine(all)

Cite this

Shcheynikov, N., Ko, S. B. H., Zeng, W., Joo, Y. C., Dorwart, M. R., Thomas, P. J., & Muallem, S. (2006). Regulatory interaction between CFTR and the SLC26 transporters. In Epithelial Anion Transport in Health and Disease: The Role of the SLC26 Transporters Family (pp. 177-186). (Novartis Foundation Symposium; Vol. 273).