Relation of cholesterol-stimulated Staphylococcus aureus growth to chronic blepharitis

W. E. Shine, R. Silvany, James P McCulley

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Purpose. Many types of chronic blepharitis have been believed to be primarily microbial in origin; however, it was proposed that differences and changes in lipid composition of meibomian secretion may be the initiating factor in some of these. It was recently reported that there are two subgroups of normals, those whose meibomian secretions contain high levels of cholesterol esters and those whose secretions contain very low levels of these esters. Thus, these subgroups of normals were defined on the basis of detailed lipid analyses of meibomian secretions from individuals showing no clinical signs of chronic blepharitis. All secretions from patients in the various disease groups contain high levels of these esters. Based on previous observations that in some chronic blepharitis disease groups certain Staphylococcus species were capable of hydrolyzing cholesterol esters, the authors tested the hypothesis that the resulting cholesterol might affect growth of Staphylococcus aureus. Methods. Staphylococcus aureus growth stimulation in Mueller-Hinton broth by cholesterol was determined by colony forming units. Growth stimulation by cholesterol and other additives was also determined by the optical density 650 nm method. Statistical analyses included analysis of variance and the Student's t test. Results. Cholesterol stimulated Staphylococcus aureus growth was significant during the first 24 hr period (20% increase at 25 μM cholesterol, P < 0.02), and for the total 48 hr period (40% increase at 400 μM cholesterol, P < 0.005) when compared to the respective control. Growth stimulation, determined by OD at 650 nm, in the presence of cholesterol was significantly greater (P < 0.02) than that in the presence of either sitosterol or cholestanol when the sterol concentration was 190 μM. Conclusion. These results suggest that the presence and hydrolysis of cholesterol esters of meibomian secretions may contribute to the proliferation of Staphylococcus spp, especially Staphylococcus aureus, observed in some chronic blepharitis disease groups.

Original languageEnglish (US)
Pages (from-to)2291-2296
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume34
Issue number7
StatePublished - 1993

Fingerprint

Blepharitis
Staphylococcus aureus
Cholesterol
Growth
Cholesterol Esters
Staphylococcus
Esters
Chronic Disease
Cholestanol
Lipids
Sterols
Analysis of Variance
Hydrolysis
Stem Cells
Students

Keywords

  • cholesterol
  • chronic blepharitis
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Relation of cholesterol-stimulated Staphylococcus aureus growth to chronic blepharitis. / Shine, W. E.; Silvany, R.; McCulley, James P.

In: Investigative Ophthalmology and Visual Science, Vol. 34, No. 7, 1993, p. 2291-2296.

Research output: Contribution to journalArticle

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abstract = "Purpose. Many types of chronic blepharitis have been believed to be primarily microbial in origin; however, it was proposed that differences and changes in lipid composition of meibomian secretion may be the initiating factor in some of these. It was recently reported that there are two subgroups of normals, those whose meibomian secretions contain high levels of cholesterol esters and those whose secretions contain very low levels of these esters. Thus, these subgroups of normals were defined on the basis of detailed lipid analyses of meibomian secretions from individuals showing no clinical signs of chronic blepharitis. All secretions from patients in the various disease groups contain high levels of these esters. Based on previous observations that in some chronic blepharitis disease groups certain Staphylococcus species were capable of hydrolyzing cholesterol esters, the authors tested the hypothesis that the resulting cholesterol might affect growth of Staphylococcus aureus. Methods. Staphylococcus aureus growth stimulation in Mueller-Hinton broth by cholesterol was determined by colony forming units. Growth stimulation by cholesterol and other additives was also determined by the optical density 650 nm method. Statistical analyses included analysis of variance and the Student's t test. Results. Cholesterol stimulated Staphylococcus aureus growth was significant during the first 24 hr period (20{\%} increase at 25 μM cholesterol, P < 0.02), and for the total 48 hr period (40{\%} increase at 400 μM cholesterol, P < 0.005) when compared to the respective control. Growth stimulation, determined by OD at 650 nm, in the presence of cholesterol was significantly greater (P < 0.02) than that in the presence of either sitosterol or cholestanol when the sterol concentration was 190 μM. Conclusion. These results suggest that the presence and hydrolysis of cholesterol esters of meibomian secretions may contribute to the proliferation of Staphylococcus spp, especially Staphylococcus aureus, observed in some chronic blepharitis disease groups.",
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AB - Purpose. Many types of chronic blepharitis have been believed to be primarily microbial in origin; however, it was proposed that differences and changes in lipid composition of meibomian secretion may be the initiating factor in some of these. It was recently reported that there are two subgroups of normals, those whose meibomian secretions contain high levels of cholesterol esters and those whose secretions contain very low levels of these esters. Thus, these subgroups of normals were defined on the basis of detailed lipid analyses of meibomian secretions from individuals showing no clinical signs of chronic blepharitis. All secretions from patients in the various disease groups contain high levels of these esters. Based on previous observations that in some chronic blepharitis disease groups certain Staphylococcus species were capable of hydrolyzing cholesterol esters, the authors tested the hypothesis that the resulting cholesterol might affect growth of Staphylococcus aureus. Methods. Staphylococcus aureus growth stimulation in Mueller-Hinton broth by cholesterol was determined by colony forming units. Growth stimulation by cholesterol and other additives was also determined by the optical density 650 nm method. Statistical analyses included analysis of variance and the Student's t test. Results. Cholesterol stimulated Staphylococcus aureus growth was significant during the first 24 hr period (20% increase at 25 μM cholesterol, P < 0.02), and for the total 48 hr period (40% increase at 400 μM cholesterol, P < 0.005) when compared to the respective control. Growth stimulation, determined by OD at 650 nm, in the presence of cholesterol was significantly greater (P < 0.02) than that in the presence of either sitosterol or cholestanol when the sterol concentration was 190 μM. Conclusion. These results suggest that the presence and hydrolysis of cholesterol esters of meibomian secretions may contribute to the proliferation of Staphylococcus spp, especially Staphylococcus aureus, observed in some chronic blepharitis disease groups.

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