Relationship between β2 microglobulin and cell surface alloantigens of the mouse

R. Geib, M. D. Poulik, E. S. Vitetta

Research output: Contribution to journalArticle

Abstract

Molecular relationships between β2m and other cell surface antigens (H 2, Tla, Ia, and Thy 1) were studied with the double immunofluorescence method. Cells were incubated with an antiserum against one antigen, capped, and then tested with an antiserum against a second antigen. Capping of β2m on thymocytes led to simultaneous capping of H 2 and Tla but not Thy 1 antigens; capping of H 2 and Tla (but not Thy 1) antigens resulted in capping of all β2m detectable by the immunofluorescence method. Similarly, capping of β2m on B or T lymphocytes resulted in capping of H 2 and vice versa. Ia antigens on B lymphocytes were not capped after the redistribution of β2m. The authors conclude from these data that, in the cell membrane of thymocytes, virtually all the β2m molecules are associated with H 2 and Tla, but not with Thy 1, and that on the cell surface of T or B lymphocytes, virtually all β2m is associated with H 2 but not with Ia. No evidence of any significant free β2m on either thymocytes or splenocytes was found.

Original languageEnglish (US)
JournalJournal of Immunology
Volume117
Issue number5
StatePublished - 1976

Fingerprint

Isoantigens
Thymocytes
Thy-1 Antigens
B-Lymphocytes
Fluorescent Antibody Technique
Immune Sera
T-Lymphocytes
Antigens
Histocompatibility Antigens Class II
Surface Antigens
Cell Membrane

ASJC Scopus subject areas

  • Immunology

Cite this

Relationship between β2 microglobulin and cell surface alloantigens of the mouse. / Geib, R.; Poulik, M. D.; Vitetta, E. S.

In: Journal of Immunology, Vol. 117, No. 5, 1976.

Research output: Contribution to journalArticle

@article{a5498db9aa5345f0b091aa306f5e5878,
title = "Relationship between β2 microglobulin and cell surface alloantigens of the mouse",
abstract = "Molecular relationships between β2m and other cell surface antigens (H 2, Tla, Ia, and Thy 1) were studied with the double immunofluorescence method. Cells were incubated with an antiserum against one antigen, capped, and then tested with an antiserum against a second antigen. Capping of β2m on thymocytes led to simultaneous capping of H 2 and Tla but not Thy 1 antigens; capping of H 2 and Tla (but not Thy 1) antigens resulted in capping of all β2m detectable by the immunofluorescence method. Similarly, capping of β2m on B or T lymphocytes resulted in capping of H 2 and vice versa. Ia antigens on B lymphocytes were not capped after the redistribution of β2m. The authors conclude from these data that, in the cell membrane of thymocytes, virtually all the β2m molecules are associated with H 2 and Tla, but not with Thy 1, and that on the cell surface of T or B lymphocytes, virtually all β2m is associated with H 2 but not with Ia. No evidence of any significant free β2m on either thymocytes or splenocytes was found.",
author = "R. Geib and Poulik, {M. D.} and Vitetta, {E. S.}",
year = "1976",
language = "English (US)",
volume = "117",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "5",

}

TY - JOUR

T1 - Relationship between β2 microglobulin and cell surface alloantigens of the mouse

AU - Geib, R.

AU - Poulik, M. D.

AU - Vitetta, E. S.

PY - 1976

Y1 - 1976

N2 - Molecular relationships between β2m and other cell surface antigens (H 2, Tla, Ia, and Thy 1) were studied with the double immunofluorescence method. Cells were incubated with an antiserum against one antigen, capped, and then tested with an antiserum against a second antigen. Capping of β2m on thymocytes led to simultaneous capping of H 2 and Tla but not Thy 1 antigens; capping of H 2 and Tla (but not Thy 1) antigens resulted in capping of all β2m detectable by the immunofluorescence method. Similarly, capping of β2m on B or T lymphocytes resulted in capping of H 2 and vice versa. Ia antigens on B lymphocytes were not capped after the redistribution of β2m. The authors conclude from these data that, in the cell membrane of thymocytes, virtually all the β2m molecules are associated with H 2 and Tla, but not with Thy 1, and that on the cell surface of T or B lymphocytes, virtually all β2m is associated with H 2 but not with Ia. No evidence of any significant free β2m on either thymocytes or splenocytes was found.

AB - Molecular relationships between β2m and other cell surface antigens (H 2, Tla, Ia, and Thy 1) were studied with the double immunofluorescence method. Cells were incubated with an antiserum against one antigen, capped, and then tested with an antiserum against a second antigen. Capping of β2m on thymocytes led to simultaneous capping of H 2 and Tla but not Thy 1 antigens; capping of H 2 and Tla (but not Thy 1) antigens resulted in capping of all β2m detectable by the immunofluorescence method. Similarly, capping of β2m on B or T lymphocytes resulted in capping of H 2 and vice versa. Ia antigens on B lymphocytes were not capped after the redistribution of β2m. The authors conclude from these data that, in the cell membrane of thymocytes, virtually all the β2m molecules are associated with H 2 and Tla, but not with Thy 1, and that on the cell surface of T or B lymphocytes, virtually all β2m is associated with H 2 but not with Ia. No evidence of any significant free β2m on either thymocytes or splenocytes was found.

UR - http://www.scopus.com/inward/record.url?scp=0017148896&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017148896&partnerID=8YFLogxK

M3 - Article

VL - 117

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 5

ER -