TY - JOUR
T1 - Relationship between energetic, ionic, and functional status in the perfused rat heart following thermal injury
T2 - A 31P and 23Na NMR study
AU - Xia, Zhao Fan
AU - Horton, Jureta W.
AU - Zhao, Piyu
AU - Lin, Carol
AU - Sherry, A. Dean
AU - Malloy, Craig R.
PY - 1997/4
Y1 - 1997/4
N2 - To test the hypothesis that energy deficits and intracellular ion derangements may be the cellular basis for intrinsic myocardial dysfunction in rats after burn trauma, we examined ATP metabolism, intracellular pH, sodium, and mechanical performance simultaneously in perfused beating hearts from sham burn or burned rats (43% TBSA, 3°scald burn, resuscitated for 24 hr with lactated Ringer's solution, Parkland formula). Intracellular calcium was also measured in myocytes harvested from parallel groups of sham burn and burn resuscitated rats. Burn trauma caused a 46% decrease in left ventricular developed pressure, a 69% decrease in +dP/dt(max) and a 72% decrease in - dP/dt(max). Intracellular to external standard sodium ratio increased (+58%) from 0.318 ± 0.027 to 0.500 ± 0.048 (P < 0.05), and intracellular calcium increased (+ 67%) from 206 ± 13 to 445 ± 37 nM (P < 0.01). Burn hearts exhibited decreased functional response to isoproterenol challenge compared to sham burn controls, but energy metabolism was similar in all hearts, regardless of burn injury. Our data suggest that burn trauma alters intracellular cardiomyocyte calcium and sodium homeostasis, and ionic derangements are not related to either altered intracellular pH or high energy phosphate deficits.
AB - To test the hypothesis that energy deficits and intracellular ion derangements may be the cellular basis for intrinsic myocardial dysfunction in rats after burn trauma, we examined ATP metabolism, intracellular pH, sodium, and mechanical performance simultaneously in perfused beating hearts from sham burn or burned rats (43% TBSA, 3°scald burn, resuscitated for 24 hr with lactated Ringer's solution, Parkland formula). Intracellular calcium was also measured in myocytes harvested from parallel groups of sham burn and burn resuscitated rats. Burn trauma caused a 46% decrease in left ventricular developed pressure, a 69% decrease in +dP/dt(max) and a 72% decrease in - dP/dt(max). Intracellular to external standard sodium ratio increased (+58%) from 0.318 ± 0.027 to 0.500 ± 0.048 (P < 0.05), and intracellular calcium increased (+ 67%) from 206 ± 13 to 445 ± 37 nM (P < 0.01). Burn hearts exhibited decreased functional response to isoproterenol challenge compared to sham burn controls, but energy metabolism was similar in all hearts, regardless of burn injury. Our data suggest that burn trauma alters intracellular cardiomyocyte calcium and sodium homeostasis, and ionic derangements are not related to either altered intracellular pH or high energy phosphate deficits.
UR - http://www.scopus.com/inward/record.url?scp=0031127799&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031127799&partnerID=8YFLogxK
U2 - 10.1006/jsre.1997.5011
DO - 10.1006/jsre.1997.5011
M3 - Article
C2 - 9202673
AN - SCOPUS:0031127799
SN - 0022-4804
VL - 69
SP - 212
EP - 219
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 1
ER -