Relationships within the family of GTP-binding proteins isolated from bovine central nervous system

D. J. Roof, M. L. Applebury, P. C. Sternweis

Research output: Contribution to journalArticle

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Abstract

Four members of a family of GTP-binding proteins (G-proteins) which translate stimulation of extracellular receptors into regulation of intracellular enzymes were isolated from the bovine central nervous system. These proteins were examined for functional similarities and cross-reactivity with antibodies to the G-protein (transductin, G(t)) from the photoreceptor system. Two proteins, G(s) and G(i), can be distinguished by their respective abilities to stimulate or inhibit adenylate cyclase. The activated α subunits of G(t) and a fourth member of the family, G(o), did not affect this enzyme. G(t) was shown to be unique in its ability to stimulate cGMP-dependent phosphodiesterase. While functionally diverse, the G-proteins were shown to have some common antigenic properties. Antibodies directed against the β subunit of G(t) recognize the β36 subunits of all preparations but not a putative second β35 subunit. Antibodies specific for the α subunit of G(t) did not recognize other α subunits when immune blots from sodium dodecyl sulfate gels were examined. However, G(o)α, but not G(s)α or G(i)α, reacted strongly with the antibodies when the native subunit was spotted directly. This suggests that G(o)α and G(t)α have homologous structural determinants. An antiserum that recognized G(t)γ did not recognize γ subunits from other sources. These data support the proposed diversity of function and similarity of structure among the four G-proteins. The α and potentially γ subunits appear to be responsible for the specificity of function.

Original languageEnglish (US)
Pages (from-to)16242-16249
Number of pages8
JournalJournal of Biological Chemistry
Volume260
Issue number30
StatePublished - 1985

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Neurology
GTP-Binding Proteins
Central Nervous System
Aptitude
Antibodies
Phosphoric Diester Hydrolases
Enzymes
Adenylyl Cyclases
Sodium Dodecyl Sulfate
Immune Sera
Proteins
Gels

ASJC Scopus subject areas

  • Biochemistry

Cite this

Relationships within the family of GTP-binding proteins isolated from bovine central nervous system. / Roof, D. J.; Applebury, M. L.; Sternweis, P. C.

In: Journal of Biological Chemistry, Vol. 260, No. 30, 1985, p. 16242-16249.

Research output: Contribution to journalArticle

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