Release of mechanical tension triggers apoptosis of human fibroblasts in a model of regressing granulation tissue

Frederick Grinnell, Meifang Zhu, Mark A. Carlson, John M. Abrams

Research output: Contribution to journalArticle

218 Scopus citations

Abstract

In an in vitro model of granulation tissue, early passage human diploid fibroblasts under mechanical tension showed little or no apoptosis. Release of mechanical tension triggered an apoptotic response that occurred within 3- 6 h and reached a plateau by 24 h. The percentage of apoptotic cells (~15%) remained constant up to 7 days, and after 3 days, total cell number declined. Identification of mechanical unloading as a stimulus for apoptosis, without application of pharmacologic or genetic intervention, is a novel observation that permits us to model similar events that occur during wound healing. Studies on the mechanism regulating apoptosis under these conditions established that the apoptotic response does not require differentiation of cells into myofibroblasts but is governed by a combination of mechanical tension and growth factors in the collagen matrix.

Original languageEnglish (US)
Pages (from-to)608-619
Number of pages12
JournalExperimental Cell Research
Volume248
Issue number2
DOIs
StatePublished - May 1 1999

Keywords

  • Apoptosis
  • Mechanical tension
  • Wound repair

ASJC Scopus subject areas

  • Cell Biology

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