Removal of hydrosalpinges increases endometrial leukaemia inhibitory factor (LIF) expression at the time of the implantation window

Emre Seli, Umit A. Kayisli, Hakan Cakmak, Orhan Bukulmez, Ibrahim Bildirici, Ozlem Guzeloglu-Kayisli, Aydin Arici

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Background: The presence of hydrosalpinges is associated with lower implantation and pregnancy rates in women undergoing IVF-embryo transfer, while salpingectomy improves these parameters. Although the mechanism by which hydrosalpinges affects fertility is not entirely understood, an adverse effect on endometrial receptivity has been postulated. In this study, we hypothesized that the adverse effects of hydrosalpinges on fertility may be in part mediated by inappropriate endometrial expression of leukaemia inhibitory factor (LIF), a cytokine implicated in implantation. Methods: In order to test our hypothesis, we prospectively examined the expression of LIF during the window of implantation in the endometrium of infertile women (n = 10) with hydrosalpinges prior to and following salpingectomy and of fertile controls (n = 10) by Western blotting and immunohistochemistry. Results: LIF expression was significantly lower in infertile women with hydrosalpinges compared with fertile controls (P < 0.05). Salpingectomy resulted in an increase in LIF expression in eight out of 10 women with hydrosalpinges. LIF levels were increased by 231 6 49% (mean 6 SEM) following salpingectomy. Immunohistochemical analysis confirmed the Western blot findings. The increased LIF immunoreactivity was predominantly localized to luminal and glandular epithelial cells. Conclusions: Our findings suggest that observed benefit from salpingectomy in infertile women with hydrosalpinges may be in part mediated by the up-regulation of endometrial LIF expression.

Original languageEnglish (US)
Pages (from-to)3012-3017
Number of pages6
JournalHuman Reproduction
Volume20
Issue number11
DOIs
StatePublished - Nov 2005

Keywords

  • Endometrial receptivity
  • Hydrosalpinx
  • Implantation
  • Leukaemia inhibitory factor
  • Salpingectomy

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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