Renal transplant patients show variations in their self-reactive repertoires

A serial study

Karina Portugal, Igor Dozmorov, Igor Sidorov, Idania Marrero, João Américo Fonseca, Mônica Spadafora-Ferreira, Jorge Kalil, Verônica Coelho

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We addressed the question of whether allo-transplantation (Tx) induces breakdown of tolerance to self-antigens or alteration of the autoreactive T cell repertoire in humans. The serial variation of T cell autoreactivity was studied in the peripheral blood of 12 renal transplant patients, by autologous limiting dilution assay and autologous mixed lymphocyte reaction. Ten of 12 patients presented a positive response in autologous peripheral blood mononuclear cells in the post-Tx period, in contrast to four of 12 patients before Tx (P = 0.038). Multi-hit kinetics was found in 57% of the assays analyzed, indicating frequent regulatory control of the autologous response. Quantitative analysis performed in eight patients showed an increase in precursor frequency at >1 year post-Tx in five patients. These data indicate that autoreactivity increases or develops following Tx, in humans. Post-Tx events such as alloreactivity, infections or immunosuppression could interfere with the balance of autoreactive and regulatory cells, leading to changes in the T cell repertoires to self-antigens and eventually breakdown of self-tolerance. Further investigation is needed to elucidate whether post-Tx autoreactivity contributes to rejection, plays a regulatory role over alloreactivity or both, at separate times.

Original languageEnglish (US)
Pages (from-to)747-755
Number of pages9
JournalInternational Immunology
Volume13
Issue number6
StatePublished - 2001

Fingerprint

Transplants
Kidney
Autoantigens
T-Lymphocytes
Self Tolerance
Mixed Lymphocyte Culture Test
Autografts
Immunosuppression
Blood Cells
Transplantation
Infection

Keywords

  • Autologous limiting dilution assay
  • Autoreactivity
  • Multi-hit limiting dilution assay
  • Regulatory cells
  • Renal transplantation

ASJC Scopus subject areas

  • Immunology

Cite this

Portugal, K., Dozmorov, I., Sidorov, I., Marrero, I., Fonseca, J. A., Spadafora-Ferreira, M., ... Coelho, V. (2001). Renal transplant patients show variations in their self-reactive repertoires: A serial study. International Immunology, 13(6), 747-755.

Renal transplant patients show variations in their self-reactive repertoires : A serial study. / Portugal, Karina; Dozmorov, Igor; Sidorov, Igor; Marrero, Idania; Fonseca, João Américo; Spadafora-Ferreira, Mônica; Kalil, Jorge; Coelho, Verônica.

In: International Immunology, Vol. 13, No. 6, 2001, p. 747-755.

Research output: Contribution to journalArticle

Portugal, K, Dozmorov, I, Sidorov, I, Marrero, I, Fonseca, JA, Spadafora-Ferreira, M, Kalil, J & Coelho, V 2001, 'Renal transplant patients show variations in their self-reactive repertoires: A serial study', International Immunology, vol. 13, no. 6, pp. 747-755.
Portugal K, Dozmorov I, Sidorov I, Marrero I, Fonseca JA, Spadafora-Ferreira M et al. Renal transplant patients show variations in their self-reactive repertoires: A serial study. International Immunology. 2001;13(6):747-755.
Portugal, Karina ; Dozmorov, Igor ; Sidorov, Igor ; Marrero, Idania ; Fonseca, João Américo ; Spadafora-Ferreira, Mônica ; Kalil, Jorge ; Coelho, Verônica. / Renal transplant patients show variations in their self-reactive repertoires : A serial study. In: International Immunology. 2001 ; Vol. 13, No. 6. pp. 747-755.
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