Renal transplant patients show variations in their self-reactive repertoires: A serial study

Karina Portugal, Igor Dozmorov, Igor Sidorov, Idania Marrero, João Américo Fonseca, Mônica Spadafora-Ferreira, Jorge Kalil, Verônica Coelho

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

We addressed the question of whether allo-transplantation (Tx) induces breakdown of tolerance to self-antigens or alteration of the autoreactive T cell repertoire in humans. The serial variation of T cell autoreactivity was studied in the peripheral blood of 12 renal transplant patients, by autologous limiting dilution assay and autologous mixed lymphocyte reaction. Ten of 12 patients presented a positive response in autologous peripheral blood mononuclear cells in the post-Tx period, in contrast to four of 12 patients before Tx (P = 0.038). Multi-hit kinetics was found in 57% of the assays analyzed, indicating frequent regulatory control of the autologous response. Quantitative analysis performed in eight patients showed an increase in precursor frequency at >1 year post-Tx in five patients. These data indicate that autoreactivity increases or develops following Tx, in humans. Post-Tx events such as alloreactivity, infections or immunosuppression could interfere with the balance of autoreactive and regulatory cells, leading to changes in the T cell repertoires to self-antigens and eventually breakdown of self-tolerance. Further investigation is needed to elucidate whether post-Tx autoreactivity contributes to rejection, plays a regulatory role over alloreactivity or both, at separate times.

Original languageEnglish (US)
Pages (from-to)747-755
Number of pages9
JournalInternational Immunology
Volume13
Issue number6
DOIs
StatePublished - Jan 1 2001

Keywords

  • Autologous limiting dilution assay
  • Autoreactivity
  • Multi-hit limiting dilution assay
  • Regulatory cells
  • Renal transplantation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Portugal, K., Dozmorov, I., Sidorov, I., Marrero, I., Fonseca, J. A., Spadafora-Ferreira, M., Kalil, J., & Coelho, V. (2001). Renal transplant patients show variations in their self-reactive repertoires: A serial study. International Immunology, 13(6), 747-755. https://doi.org/10.1093/intimm/13.6.747