Repair of nitrous acid damage to DNA in Escherichia coli

Ricardo Da Roza, Errol C. Friedberg, Bruce K. Duncan, Huber R. Warner

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A number of mutant strains of Escherichia coli have been examined for their sensitivity to nitrous acid and in some instances to methylmethanesulfonate. All ung- mutants tested are abnormally sensitive to nitrous acid. Since the ung mutation is phenotypically expressed as a defect in uracil DNA glycosidase, this observation supports the contention that treatment of cells with nitrous acid causes deamination of cytosine to uracil. In addition the observed sentitivity indicates that the ung gene is involved in the repair of uracil in DNA. Studies with other mutants suggest that both exonuclease III and DNA polymerase I of E. coli are involved in the repair of nitrous acid damage in vivo.

Original languageEnglish (US)
Pages (from-to)4934-4939
Number of pages6
JournalBiochemistry
Volume16
Issue number22
StatePublished - 1977

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Nitrous Acid
Escherichia coli
DNA Damage
Repair
Uracil
DNA
Uracil-DNA Glycosidase
DNA Polymerase I
Deamination
Cytosine
Genes
Cells
Defects
Mutation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Da Roza, R., Friedberg, E. C., Duncan, B. K., & Warner, H. R. (1977). Repair of nitrous acid damage to DNA in Escherichia coli. Biochemistry, 16(22), 4934-4939.

Repair of nitrous acid damage to DNA in Escherichia coli. / Da Roza, Ricardo; Friedberg, Errol C.; Duncan, Bruce K.; Warner, Huber R.

In: Biochemistry, Vol. 16, No. 22, 1977, p. 4934-4939.

Research output: Contribution to journalArticle

Da Roza, R, Friedberg, EC, Duncan, BK & Warner, HR 1977, 'Repair of nitrous acid damage to DNA in Escherichia coli', Biochemistry, vol. 16, no. 22, pp. 4934-4939.
Da Roza R, Friedberg EC, Duncan BK, Warner HR. Repair of nitrous acid damage to DNA in Escherichia coli. Biochemistry. 1977;16(22):4934-4939.
Da Roza, Ricardo ; Friedberg, Errol C. ; Duncan, Bruce K. ; Warner, Huber R. / Repair of nitrous acid damage to DNA in Escherichia coli. In: Biochemistry. 1977 ; Vol. 16, No. 22. pp. 4934-4939.
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