Reparative myocardial mechanisms in adult C57BL/6 and MRL mice following injury

R. Haris Naseem, Annette P. Meeson, J. Michael DiMaio, Michael D. White, Justin Kallhoff, Caroline Humphries, Sean C. Goetsch, Leon J. De Windt, Maurice A. Williams, Mary G. Garry, Daniel J. Garry

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Previous studies have suggested that the heart may be capable of limited repair and regeneration in response to a focal injury, while other studies indicate that the mammalian heart has no regenerative capacity. To further explore this issue, we performed a series of superficial and transmural myocardial injuries in C57BL/6 and MRL/MpJ adult mice. At defined time intervals following the respective injury (days 3, 14, 30 and 60), we examined cardiac function using echocardiography, morphology, fluorescence-activated cell sorting for 5-bromo-2-deoxyuridine-positive cells and molecular signature using microarray analysis. We observed restoration of myocardial function in the superficial MRL cryoinjured heart and significantly less collagen deposition compared with the injured hearts of C57BL/6 mice. Following a severe transmural myocardial injury, the MRL mouse has increased survival and decreased ventricular remodeling compared with the C57BL/6 mouse but without evidence of complete regeneration. The cytoprotective program observed in the severely injured MRL heart is in part due to increased cellular proliferation, increased vasculogenesis, and decreased apoptosis that limits the extension of the injury. We conclude that MRL injured hearts have evidence of myocardial regeneration, in response to superficial injury, but the stabilized left ventricular function and improved survival observed in the MRL mouse following severe injury is not associated with complete myocardial regeneration.

Original languageEnglish (US)
Pages (from-to)44-52
Number of pages9
JournalPhysiological Genomics
Volume30
Issue number1
DOIs
StatePublished - Jun 19 2007

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Wounds and Injuries
Regeneration
Inbred C57BL Mouse
Ventricular Remodeling
Bromodeoxyuridine
Microarray Analysis
Left Ventricular Function
Echocardiography
Flow Cytometry
Collagen
Cell Proliferation
Apoptosis

Keywords

  • Cytoprotection
  • Echocardiography
  • Myocardial regeneration
  • Progenitor cells
  • Transcriptome analysis
  • TUNEL assay

ASJC Scopus subject areas

  • Physiology
  • Genetics

Cite this

Naseem, R. H., Meeson, A. P., DiMaio, J. M., White, M. D., Kallhoff, J., Humphries, C., ... Garry, D. J. (2007). Reparative myocardial mechanisms in adult C57BL/6 and MRL mice following injury. Physiological Genomics, 30(1), 44-52. https://doi.org/10.1152/physiolgenomics.00070.2006

Reparative myocardial mechanisms in adult C57BL/6 and MRL mice following injury. / Naseem, R. Haris; Meeson, Annette P.; DiMaio, J. Michael; White, Michael D.; Kallhoff, Justin; Humphries, Caroline; Goetsch, Sean C.; De Windt, Leon J.; Williams, Maurice A.; Garry, Mary G.; Garry, Daniel J.

In: Physiological Genomics, Vol. 30, No. 1, 19.06.2007, p. 44-52.

Research output: Contribution to journalArticle

Naseem, RH, Meeson, AP, DiMaio, JM, White, MD, Kallhoff, J, Humphries, C, Goetsch, SC, De Windt, LJ, Williams, MA, Garry, MG & Garry, DJ 2007, 'Reparative myocardial mechanisms in adult C57BL/6 and MRL mice following injury', Physiological Genomics, vol. 30, no. 1, pp. 44-52. https://doi.org/10.1152/physiolgenomics.00070.2006
Naseem RH, Meeson AP, DiMaio JM, White MD, Kallhoff J, Humphries C et al. Reparative myocardial mechanisms in adult C57BL/6 and MRL mice following injury. Physiological Genomics. 2007 Jun 19;30(1):44-52. https://doi.org/10.1152/physiolgenomics.00070.2006
Naseem, R. Haris ; Meeson, Annette P. ; DiMaio, J. Michael ; White, Michael D. ; Kallhoff, Justin ; Humphries, Caroline ; Goetsch, Sean C. ; De Windt, Leon J. ; Williams, Maurice A. ; Garry, Mary G. ; Garry, Daniel J. / Reparative myocardial mechanisms in adult C57BL/6 and MRL mice following injury. In: Physiological Genomics. 2007 ; Vol. 30, No. 1. pp. 44-52.
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