Reperfusion enhances the local release of endothelin after regional myocardial ischemia

Carlos E. Velasco, Michael Turner, Tadashi Inagami, James B. Atkinson, Renu Virmani, Edwin K. Jackson, John J. Murray, Mervyn B. Forman

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Abstract

The role of endothelin, a potent long-acting vasoconstrictor peptide, in the pathogenesis of the "no-reflow" phenomenon was investigated in nine closed-chest mongrel dogs undergoing 90 minutes of proximal left anterior descending artery occlusion and 3.5 hours of reperfusion. Endothelin levels were measured serially from the coronary sinus (CS) and aorta (Ao) by radioimmunoassay and correlated with regional myocardial blood perfusion. Prolonged anesthesia, surgery, and vascular instrumentation did not change endothelin levels in four sham animals. A progressive and parallel increase in CS and Ao endothelin levels occurred during coronary occlusion. A further increase in CS levels was observed during the reperfusion period, resulting in significantly higher values of the peptide at 30 and 60 minutes (30 minutes: CS 22.1 ± 3.5 vs Ao 15.1 ± 5.1 pg/ml; 60 minutes: CS 21.1 ± 4.5 vs Ao 15.0 ± 3.6 pg/ml; p < 0.05 by analysis of variance). Microvascular perfusion determined semiquantatively with fluorescent beads was significantly reduced in the central ischemic zone (CIZ) compared with that in the nonischemic zone (NIZ) (CIZ endocardium 1.14 ± 0.4 beads/m2, CIZ midmyocardium 1.19 ± 0.3 beads/m2, NIZ 3.8 ± 0.6 beads/m2; p < 0.05). A significant correlation was noted between mean reperfusion levels of endothelin in the CS and endocardial flow in the CIZ (r = -0.88; p = 0.009). This study demonstrates that reperfusion per se enhanced the spillover of endothelin from the cardiac interstitium. Local release of endothelin may contribute to the progressive decrease in microvascular flow in the reperfused bed.

Original languageEnglish (US)
Pages (from-to)441-451
Number of pages11
JournalAmerican Heart Journal
Volume128
Issue number3
DOIs
StatePublished - Jan 1 1994

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Endothelins
Coronary Sinus
Reperfusion
Myocardial Ischemia
Aorta
Perfusion
No-Reflow Phenomenon
Endocardium
Peptides
Coronary Occlusion
Vasoconstrictor Agents
Radioimmunoassay
Blood Vessels
Analysis of Variance
Thorax
Anesthesia
Arteries
Dogs

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Velasco, C. E., Turner, M., Inagami, T., Atkinson, J. B., Virmani, R., Jackson, E. K., ... Forman, M. B. (1994). Reperfusion enhances the local release of endothelin after regional myocardial ischemia. American Heart Journal, 128(3), 441-451. https://doi.org/10.1016/0002-8703(94)90615-7

Reperfusion enhances the local release of endothelin after regional myocardial ischemia. / Velasco, Carlos E.; Turner, Michael; Inagami, Tadashi; Atkinson, James B.; Virmani, Renu; Jackson, Edwin K.; Murray, John J.; Forman, Mervyn B.

In: American Heart Journal, Vol. 128, No. 3, 01.01.1994, p. 441-451.

Research output: Contribution to journalArticle

Velasco, CE, Turner, M, Inagami, T, Atkinson, JB, Virmani, R, Jackson, EK, Murray, JJ & Forman, MB 1994, 'Reperfusion enhances the local release of endothelin after regional myocardial ischemia', American Heart Journal, vol. 128, no. 3, pp. 441-451. https://doi.org/10.1016/0002-8703(94)90615-7
Velasco CE, Turner M, Inagami T, Atkinson JB, Virmani R, Jackson EK et al. Reperfusion enhances the local release of endothelin after regional myocardial ischemia. American Heart Journal. 1994 Jan 1;128(3):441-451. https://doi.org/10.1016/0002-8703(94)90615-7
Velasco, Carlos E. ; Turner, Michael ; Inagami, Tadashi ; Atkinson, James B. ; Virmani, Renu ; Jackson, Edwin K. ; Murray, John J. ; Forman, Mervyn B. / Reperfusion enhances the local release of endothelin after regional myocardial ischemia. In: American Heart Journal. 1994 ; Vol. 128, No. 3. pp. 441-451.
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