TY - JOUR
T1 - Requirement for metalloendoprotease in exocytosis
T2 - Evidence in mast cells and adrenal chromaffin cells
AU - Mundy, Dorothy I.
AU - Strittmatter, Warren J.
N1 - Funding Information:
This work was supported by a Teacher-Investigator Award from the National Institute of Neurological and Communicative Disorders and Stroke. We would like to thank Dr. Henry Epstein for his advice. We would like to thank Dr. Jack Waymire for the chromaffin cell culture technology and valuable discussions and Robert Akridge for preparation of the dissociated cultures.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1985/3
Y1 - 1985/3
N2 - Exocytosis is initiated by the receptor-mediated influx of calcium that results in fusion of the secretory vesicle with the plasma membrane. We examined the possibility that calcium-dependent exocytosis in mast cells and adrenal chromaffin cells requires metalloendoprotease activity. Metalloendoprotease inhibitors and dipeptide substrates block exocytosis in these cells with the same specificity and dose dependency as that with which they interact with metalloendoproteases. Metalloendoprotease activity is identified in these cells with fluorogenic synthetic substrates, which also blocked exocytosis. Metalloendoprotease activity is highest in the plasma membrane of chromaffin cells. The metalloendoprotease appears to be required in exocytosis at a step dependent on or after calcium entry, since exocytosis initiated by direct calcium introduction in both mast cells and chromaffin cells is blocked by metalloendoprotease inhibitors.
AB - Exocytosis is initiated by the receptor-mediated influx of calcium that results in fusion of the secretory vesicle with the plasma membrane. We examined the possibility that calcium-dependent exocytosis in mast cells and adrenal chromaffin cells requires metalloendoprotease activity. Metalloendoprotease inhibitors and dipeptide substrates block exocytosis in these cells with the same specificity and dose dependency as that with which they interact with metalloendoproteases. Metalloendoprotease activity is identified in these cells with fluorogenic synthetic substrates, which also blocked exocytosis. Metalloendoprotease activity is highest in the plasma membrane of chromaffin cells. The metalloendoprotease appears to be required in exocytosis at a step dependent on or after calcium entry, since exocytosis initiated by direct calcium introduction in both mast cells and chromaffin cells is blocked by metalloendoprotease inhibitors.
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U2 - 10.1016/0092-8674(85)90213-2
DO - 10.1016/0092-8674(85)90213-2
M3 - Article
C2 - 2578889
AN - SCOPUS:0021846078
SN - 0092-8674
VL - 40
SP - 645
EP - 656
JO - Cell
JF - Cell
IS - 3
ER -