Requirement for metalloendoprotease in exocytosis: Evidence in mast cells and adrenal chromaffin cells

Dorothy I. Mundy, Warren J. Strittmatter

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

Exocytosis is initiated by the receptor-mediated influx of calcium that results in fusion of the secretory vesicle with the plasma membrane. We examined the possibility that calcium-dependent exocytosis in mast cells and adrenal chromaffin cells requires metalloendoprotease activity. Metalloendoprotease inhibitors and dipeptide substrates block exocytosis in these cells with the same specificity and dose dependency as that with which they interact with metalloendoproteases. Metalloendoprotease activity is identified in these cells with fluorogenic synthetic substrates, which also blocked exocytosis. Metalloendoprotease activity is highest in the plasma membrane of chromaffin cells. The metalloendoprotease appears to be required in exocytosis at a step dependent on or after calcium entry, since exocytosis initiated by direct calcium introduction in both mast cells and chromaffin cells is blocked by metalloendoprotease inhibitors.

Original languageEnglish (US)
Pages (from-to)645-656
Number of pages12
JournalCell
Volume40
Issue number3
DOIs
StatePublished - Mar 1985

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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