TY - JOUR
T1 - Research resource
T2 - Comprehensive expression atlas of the fibroblast growth factor system in adult mouse
AU - Tacer, Klementina Fon
AU - Bookout, Angie L.
AU - Ding, Xunshan
AU - Kurosu, Hiroshi
AU - John, George B.
AU - Wang, Lei
AU - Goetz, Regina
AU - Mohammadi, Moosa
AU - Kuro-o, Makoto
AU - Mangelsdorf, David J.
AU - Kliewer, Steven A.
PY - 2010/10
Y1 - 2010/10
N2 - Although members of the fibroblast growth factor (FGF) family and their receptors have well-established roles in embryogenesis, their contributions to adult physiology remain relatively unexplored. Here, we use real-time quantitative PCR to determine the mRNA expression patterns of all 22 FGFs, the seven principal FGF receptors (FGFRs), and the three members of the Klotho family of coreceptors in 39 different mouse tissues. Unsupervised hierarchical cluster analysis of the mRNA expression data reveals that most FGFs and FGFRs fall into two groups the expression of which is enriched in either the central nervous system or reproductive and gastrointestinal tissues. Interestingly, the FGFs that can act as endocrine hormones, including FGF15/19, FGF21, and FGF23, cluster in a third group that does not include any FGFRs, underscoring their roles in signaling between tissues. We further show that the most recently identified Klotho family member, Lactase-like, is highly and selectively expressed in brown adipose tissue and eye and can function as an additional coreceptor for FGF19. This FGF atlas provides an important resource for guiding future studies to elucidate the physiological functions of FGFs in adult animals.
AB - Although members of the fibroblast growth factor (FGF) family and their receptors have well-established roles in embryogenesis, their contributions to adult physiology remain relatively unexplored. Here, we use real-time quantitative PCR to determine the mRNA expression patterns of all 22 FGFs, the seven principal FGF receptors (FGFRs), and the three members of the Klotho family of coreceptors in 39 different mouse tissues. Unsupervised hierarchical cluster analysis of the mRNA expression data reveals that most FGFs and FGFRs fall into two groups the expression of which is enriched in either the central nervous system or reproductive and gastrointestinal tissues. Interestingly, the FGFs that can act as endocrine hormones, including FGF15/19, FGF21, and FGF23, cluster in a third group that does not include any FGFRs, underscoring their roles in signaling between tissues. We further show that the most recently identified Klotho family member, Lactase-like, is highly and selectively expressed in brown adipose tissue and eye and can function as an additional coreceptor for FGF19. This FGF atlas provides an important resource for guiding future studies to elucidate the physiological functions of FGFs in adult animals.
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U2 - 10.1210/me.2010-0142
DO - 10.1210/me.2010-0142
M3 - Article
C2 - 20667984
AN - SCOPUS:77957376253
SN - 0888-8809
VL - 24
SP - 2050
EP - 2064
JO - Molecular Endocrinology
JF - Molecular Endocrinology
IS - 10
ER -