TY - JOUR
T1 - Research Techniques Made Simple
T2 - Forward Genetic Screening to Uncover Genes Involved in Skin Biology
AU - McAlpine, William
AU - Russell, J.
AU - Murray, Anne R.
AU - Beutler, B.
AU - Turer, Emre
N1 - Funding Information:
We thank Diantha Lavine for expert assistance in figure generation. This work was supported by the Immunology T32 Training Grant AI005184 at the UT Southwestern Medical Center (to WM); NIH Grants K08 DK107886 (to ET), R37 GM066759 (to BB), R01 AI125581 (to BB), and U19 AI100627 (to BB); and by the Lyda Hill Foundation.
Publisher Copyright:
© 2019 The Authors
PY - 2019/9
Y1 - 2019/9
N2 - The primary goals of modern genetics are to identify disease-causing mutations and to define the functions of genes in biological processes. Two complementary approaches, reverse and forward genetics, can be used to achieve this goal. Reverse genetics is a gene-driven approach that comprises specific gene targeting followed by phenotypic assessment. Conversely, forward genetics is a phenotype-driven approach that involves the phenotypic screening of organisms with randomly induced mutations followed by subsequent identification of the causative mutations (i.e., those responsible for phenotype). In this article, we focus on how forward genetics in mice can be used to explore dermatologic disease. We outline mouse mutagenesis with the chemical N-ethyl-N-nitrosourea and the strategy used to instantaneously identify mutations that are causative of specific phenotypes. Furthermore, we summarize the types of phenotypic screens that can be performed to explore various aspects of dermatologic disease.
AB - The primary goals of modern genetics are to identify disease-causing mutations and to define the functions of genes in biological processes. Two complementary approaches, reverse and forward genetics, can be used to achieve this goal. Reverse genetics is a gene-driven approach that comprises specific gene targeting followed by phenotypic assessment. Conversely, forward genetics is a phenotype-driven approach that involves the phenotypic screening of organisms with randomly induced mutations followed by subsequent identification of the causative mutations (i.e., those responsible for phenotype). In this article, we focus on how forward genetics in mice can be used to explore dermatologic disease. We outline mouse mutagenesis with the chemical N-ethyl-N-nitrosourea and the strategy used to instantaneously identify mutations that are causative of specific phenotypes. Furthermore, we summarize the types of phenotypic screens that can be performed to explore various aspects of dermatologic disease.
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U2 - 10.1016/j.jid.2019.04.013
DO - 10.1016/j.jid.2019.04.013
M3 - Short survey
C2 - 31445571
AN - SCOPUS:85070560336
SN - 0022-202X
VL - 139
SP - 1848-1853.e1
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 9
ER -