Residues in pockets B and F of HLA-B27 are critical in the presentation of an influenza A virus nucleoprotein peptide and influence the stability of peptide-MHC complexes

Beatriz M. Carreno, Christine C. Winter, Joel D. Taurog, Ted H. Hansen, William E. Biddison

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Six pockets, designated A through F, which extend from the peptide binding site of class I HLA molecules, have been postulated to play an important role in determining peptide binding specificity. HLA-B27 mutant molecules with single amino acid substitutions at residues 9his-phe in 24thr←ser, 45glu←thr, and 67cys←ala in pocket B; 114his←asn in pocket D; and 116asp←phe in pocket F have been generated and characterized for their capacity to present an influenza A nucleoprotein peptide (NP 383-391) for cytotoxic T iymphocyte recognition. We report here that substitutions in residues 45, 67, and 116 affect presentation of NP 383-391 when peptide is processed and loaded during viral infection. Using 125l-labeled NP peptide, we demonstrate that substitutions in residues 67 and 116 alter the stability of NP-HLA-B27 complexes. A substitution at position 9 of the NP peptide complements the mutation introduced at residue 116, suggesting that the NP peptide binds with its carboxy terminal amino acid in pocket F. These findings indicate that polymorphic residues within pockets B and F of HLA-B27 play a crucial role in peptide binding and stability of peptide-MHC class I complexes. Furthermore, our results suggest that substitutions at allele-speciflc residues within pockets B and F alter the stability of NP-HLA-B27 complexes resulting in the diminution or abrogation of NP presentation during viral infection.

Original languageEnglish (US)
Pages (from-to)353-360
Number of pages8
JournalInternational Immunology
Volume5
Issue number4
DOIs
StatePublished - Dec 1 1993

Keywords

  • Antigen presentation
  • HLA-B27
  • MHC class I

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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