Resistance to ischemic damage in hearts of starved rabbits. Correlation with lysosomal alterations and delayed release of cathepsin D

R. S. Decker, J. S. Crie, A. R. Poole, J. T. Dingle, K. Wildenthal

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Prolonged starvation produces dramatic changes both in the lysosomal properties of the heart and in its energy stores and, therefore, might be expected to alter some of the characteristic cardiac responses to ischemia. To test this possiblility we ligated the circumflex coronary artery of rabbits that had been fed normally or starved for 6 days. Ultrastructural evidence of myocytic damage following 30 to 120 minutes of ischemia was much less severe in the starved animals than in the normally fed group. The development of signs of irreversible injury (e.g. osmiophilic densities in mitochondria) was delayed for 1 hour or more by starvation. A similar delay occurred in the biochemical redistribution of cathespin D activity and in the cytoplasmic release of acid hydrolases from lysosomes and sarcoplasmic reticulum. These results indicate a marked protective effect of starvation against myocardial ischemia. In addition, both in starved and in fed animals, ischemically induced release of lysosomal enzymes was closely linked temporally to the development of subcellular damage.

Original languageEnglish (US)
Pages (from-to)197-207
Number of pages11
JournalLaboratory Investigation
Volume43
Issue number3
StatePublished - 1980

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Cathepsin D
Starvation
Rabbits
Ischemia
Sarcoplasmic Reticulum
Hydrolases
Lysosomes
Myocardial Ischemia
Coronary Vessels
Mitochondria
Acids
Wounds and Injuries
Enzymes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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Resistance to ischemic damage in hearts of starved rabbits. Correlation with lysosomal alterations and delayed release of cathepsin D. / Decker, R. S.; Crie, J. S.; Poole, A. R.; Dingle, J. T.; Wildenthal, K.

In: Laboratory Investigation, Vol. 43, No. 3, 1980, p. 197-207.

Research output: Contribution to journalArticle

Decker, R. S. ; Crie, J. S. ; Poole, A. R. ; Dingle, J. T. ; Wildenthal, K. / Resistance to ischemic damage in hearts of starved rabbits. Correlation with lysosomal alterations and delayed release of cathepsin D. In: Laboratory Investigation. 1980 ; Vol. 43, No. 3. pp. 197-207.
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