Resolution of dihydroxyeicosanoates and of dihydroxyeicosatrienoates by chiral phase chromatography

Jorge H. Capdevila, Shouzou Wei, Anil Kumar, Jun Kobayashi, James R. Snapper, Darryl C. Zeldin, Rama K. Bhatt, J R Falck

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

A chromatographic method is described for the direct enantiomeric characterization of 5,6-, 8,9-, 11,12-, and 14,15-vic-dihydroxyeicosatrienoic acids (DHETs), metabolites of the cytochrome P-450 arachidonate epoxygenase pathway, and of their corresponding saturated vic-dihydroxyeicosanoic acids. Following esterification, the individual methyl or pentafluorobenzyl esters are resolved by chiral-phase chromatography utilizing a Chiralcel OC or OD column. This methodology will find analytical and preparative applications since it is simple and efficient and preserves, intact, the diol functionality.

Original languageEnglish (US)
Pages (from-to)236-240
Number of pages5
JournalAnalytical biochemistry
Volume207
Issue number2
DOIs
StatePublished - Dec 1992

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Capdevila, J. H., Wei, S., Kumar, A., Kobayashi, J., Snapper, J. R., Zeldin, D. C., Bhatt, R. K., & Falck, J. R. (1992). Resolution of dihydroxyeicosanoates and of dihydroxyeicosatrienoates by chiral phase chromatography. Analytical biochemistry, 207(2), 236-240. https://doi.org/10.1016/0003-2697(92)90006-S