TY - JOUR
T1 - Resolution of dihydroxyeicosanoates and of dihydroxyeicosatrienoates by chiral phase chromatography
AU - Capdevila, Jorge H.
AU - Wei, Shouzou
AU - Kumar, Anil
AU - Kobayashi, Jun
AU - Snapper, James R.
AU - Zeldin, Darryl C.
AU - Bhatt, Rama K.
AU - Falck, J R
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1992/12
Y1 - 1992/12
N2 - A chromatographic method is described for the direct enantiomeric characterization of 5,6-, 8,9-, 11,12-, and 14,15-vic-dihydroxyeicosatrienoic acids (DHETs), metabolites of the cytochrome P-450 arachidonate epoxygenase pathway, and of their corresponding saturated vic-dihydroxyeicosanoic acids. Following esterification, the individual methyl or pentafluorobenzyl esters are resolved by chiral-phase chromatography utilizing a Chiralcel OC or OD column. This methodology will find analytical and preparative applications since it is simple and efficient and preserves, intact, the diol functionality.
AB - A chromatographic method is described for the direct enantiomeric characterization of 5,6-, 8,9-, 11,12-, and 14,15-vic-dihydroxyeicosatrienoic acids (DHETs), metabolites of the cytochrome P-450 arachidonate epoxygenase pathway, and of their corresponding saturated vic-dihydroxyeicosanoic acids. Following esterification, the individual methyl or pentafluorobenzyl esters are resolved by chiral-phase chromatography utilizing a Chiralcel OC or OD column. This methodology will find analytical and preparative applications since it is simple and efficient and preserves, intact, the diol functionality.
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U2 - 10.1016/0003-2697(92)90006-S
DO - 10.1016/0003-2697(92)90006-S
M3 - Article
C2 - 1481976
AN - SCOPUS:0026968885
SN - 0003-2697
VL - 207
SP - 236
EP - 240
JO - Analytical biochemistry
JF - Analytical biochemistry
IS - 2
ER -