TY - JOUR
T1 - Resolving cellular and molecular diversity along the hippocampal anterior-to-posterior axis in humans
AU - Ayhan, Fatma
AU - Kulkarni, Ashwinikumar
AU - Berto, Stefano
AU - Sivaprakasam, Karthigayini
AU - Douglas, Connor
AU - Lega, Bradley C.
AU - Konopka, Genevieve
N1 - Publisher Copyright:
© 2021 Elsevier Inc.
PY - 2021/7/7
Y1 - 2021/7/7
N2 - The hippocampus supports many facets of cognition, including learning, memory, and emotional processing. Anatomically, the hippocampus runs along a longitudinal axis, posterior to anterior in primates. The structure, function, and connectivity of the hippocampus vary along this axis. In human hippocampus, longitudinal functional heterogeneity remains an active area of investigation, and structural heterogeneity has not been described. To understand the cellular and molecular diversity along the hippocampal long axis in human brain and define molecular signatures corresponding to functional domains, we performed single-nuclei RNA sequencing on surgically resected human anterior and posterior hippocampus from epilepsy patients, identifying differentially expressed genes at cellular resolution. We further identify axis- and cell-type-specific gene expression signatures that differentially intersect with human genetic signals, identifying cell-type-specific genes in the posterior hippocampus for cognitive function and the anterior hippocampus for mood and affect. These data are accessible as a public resource through an interactive website.
AB - The hippocampus supports many facets of cognition, including learning, memory, and emotional processing. Anatomically, the hippocampus runs along a longitudinal axis, posterior to anterior in primates. The structure, function, and connectivity of the hippocampus vary along this axis. In human hippocampus, longitudinal functional heterogeneity remains an active area of investigation, and structural heterogeneity has not been described. To understand the cellular and molecular diversity along the hippocampal long axis in human brain and define molecular signatures corresponding to functional domains, we performed single-nuclei RNA sequencing on surgically resected human anterior and posterior hippocampus from epilepsy patients, identifying differentially expressed genes at cellular resolution. We further identify axis- and cell-type-specific gene expression signatures that differentially intersect with human genetic signals, identifying cell-type-specific genes in the posterior hippocampus for cognitive function and the anterior hippocampus for mood and affect. These data are accessible as a public resource through an interactive website.
KW - hippocampus, single-cell genomics, cognition, memory, neurogenomics
UR - http://www.scopus.com/inward/record.url?scp=85109200513&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85109200513&partnerID=8YFLogxK
U2 - 10.1016/j.neuron.2021.05.003
DO - 10.1016/j.neuron.2021.05.003
M3 - Article
C2 - 34051145
AN - SCOPUS:85109200513
SN - 0896-6273
VL - 109
SP - 2091-2105.e6
JO - Neuron
JF - Neuron
IS - 13
ER -