Resonance transduction of low level periodic signals by an enzyme: An oscillatory activation barrier model

V. S. Markin, D. Liu, M. D. Rosenberg, T. Y. Tsong

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The overall rate of an enzyme catalyzed reaction is determined by the activation barrier of a rate-limiting step. If the barrier is oscillatory due to the intrinsic properties of a fluctuating enzyme, this enzymic reaction will be influenced by a low level periodic electric field through the resonance transduction between the applied field and the oscillatory activation barrier. The ATP hydrolysis activity of a highly purified, detergent solubilized Ecto-ATPase from chicken oviduct was used to test the above concept. At 37°C, this activity (1,800 μmol mg-1 min-1) was stimulated up to 47% (to 2,650 μmol mg-1 min-1) by an alternating electric field (AC), with a frequency window at 10 kHz. The maximal stimulation occurred at 5.0 V (peak-to-peak) cm-1. The potential drop across the dimension of the enzyme was ~10 μV (micelle diameter 20 nm). The activation barrier, or the Arrhenius activation energy, of the ATP splitting was measured to be 30 kT and the maximal barrier oscillation was calculated to be ~2.5 kT according to the oscillatory activation barrier (OAB) model. With the optimal AC field, full impact of the electric stimulation could be effected in much less than a second. The OAB model is many orders of magnitude more sensitive for deciphering low level periodic signals than the electroconformational coupling (ECC) model, although the latter has the ability to actively transduce energy while the former does not. By the OAB mechanism, the detecting limit of an external electric field by the ATPase, in a cell 20 μm in diameter, would be 5 mV cm-1, butcould be much lower for other membrane enzymes or receptors (e.g., nV cm-1). We propose that mechanisms similar to the OAB model could explain how a weak electromagnetic field or acoustic noises can exert its effects on an organism or a living cell.

Original languageEnglish (US)
Pages (from-to)1045-1049
Number of pages5
JournalBiophysical Journal
Volume61
Issue number4
StatePublished - 1992

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Enzymes
Adenosine Triphosphate
Electromagnetic Fields
Oviducts
Micelles
Acoustics
Detergents
Electric Stimulation
Adenosine Triphosphatases
Noise
Chickens
Hydrolysis
Membranes
ectoATPase

ASJC Scopus subject areas

  • Biophysics

Cite this

Markin, V. S., Liu, D., Rosenberg, M. D., & Tsong, T. Y. (1992). Resonance transduction of low level periodic signals by an enzyme: An oscillatory activation barrier model. Biophysical Journal, 61(4), 1045-1049.

Resonance transduction of low level periodic signals by an enzyme : An oscillatory activation barrier model. / Markin, V. S.; Liu, D.; Rosenberg, M. D.; Tsong, T. Y.

In: Biophysical Journal, Vol. 61, No. 4, 1992, p. 1045-1049.

Research output: Contribution to journalArticle

Markin, VS, Liu, D, Rosenberg, MD & Tsong, TY 1992, 'Resonance transduction of low level periodic signals by an enzyme: An oscillatory activation barrier model', Biophysical Journal, vol. 61, no. 4, pp. 1045-1049.
Markin, V. S. ; Liu, D. ; Rosenberg, M. D. ; Tsong, T. Y. / Resonance transduction of low level periodic signals by an enzyme : An oscillatory activation barrier model. In: Biophysical Journal. 1992 ; Vol. 61, No. 4. pp. 1045-1049.
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