Responsiveness of human carcinoma cells of gynecologic origin to 1,25-dihydroxycholecalciferol

Wayne A. Christopherson, John C. Porter, Paul C. MacDonald, M. Linette Casey

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

In responsive tissues the induction of 25-hydroxycholecalciferol 24-hydroxylase activity is a marker of 1,25-dihydroxycholecalciferol action. In this study we investigated the responsiveness of various malignant cells, which were derived from carcinomas of gynecologic origin, to 1,25-dihydroxycholecalciferol; we did so because it has been demonstrated that replication of certain malignant human cells that are 1,25-dihydroxycholecalciferol-responsive is inhibited by treatment with this substance. We found that 1,25-dihydroxycholecalciferol treatment caused an increase in 25-hydroxycholecalciferol 24-hydroxylase activity in gynecologic carcinoma cells that were maintained in monolayer culture. The cells were established from ovarian, endometrial, cervical, and vaginal neoplasms. After treatment with 1,25-dihydroxycholecalciferol (10 nmol/L) for 18 hours, the specific activity of 25-hydroxycholecalciferol 24-hydroxylase increased between 2.4- and 49-fold in these cells. This increase in 25-hydroxycholecalciferol 24-hydroxylase activity is suggestive that certain gynecologic malignancies are responsive to 1,25-dihydroxycholecalciferol. We also found, however, that 1,25-dihydroxycholecalciferol did not act to decrease the rate of replication of these cells; indeed in ovarian adenocarcinoma cells, 1,25-dihydroxycholecalciferol caused a decrease in doubling time; in the other cells of this study, there was no effect of 1,25-dihydroxycholecalciferol on cell replication.

Original languageEnglish (US)
Pages (from-to)1293-1296
Number of pages4
JournalAmerican journal of obstetrics and gynecology
Volume155
Issue number6
DOIs
StatePublished - Dec 1986

Keywords

  • 24-hydroxylase
  • Vitamin D
  • gynecologic malignancy

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Fingerprint Dive into the research topics of 'Responsiveness of human carcinoma cells of gynecologic origin to 1,25-dihydroxycholecalciferol'. Together they form a unique fingerprint.

  • Cite this