REST regulation of gene networks in adult neural stem cells

Shradha Mukherjee, Rebecca Brulet, Ling Zhang, Jenny Hsieh

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Adult hippocampal neural stem cells generate newborn neurons throughout life due to their ability to self-renew and exist as quiescent neural progenitors (QNPs) before differentiating into transit-amplifying progenitors (TAPs) and newborn neurons. The mechanisms that control adult neural stem cell self-renewal are still largely unknown. Conditional knockout of REST (repressor element 1-silencing transcription factor) results in precocious activation of QNPs and reduced neurogenesis over time. To gain insight into the molecular mechanisms by which REST regulates adult neural stem cells, we perform chromatin immunoprecipitation sequencing and RNA-sequencing to identify direct REST target genes. We find REST regulates both QNPs and TAPs, and importantly, ribosome biogenesis, cell cycle and neuronal genes in the process. Furthermore, overexpression of individual REST target ribosome biogenesis or cell cycle genes is sufficient to induce activation of QNPs. Our data define novel REST targets to maintain the quiescent neural stem cell state.

Original languageEnglish (US)
Article number13360
JournalNature Communications
Volume7
DOIs
StatePublished - Nov 7 2016

Fingerprint

Transcriptional Silencer Elements
Adult Stem Cells
Neural Stem Cells
stem cells
Gene Regulatory Networks
Stem cells
genes
Transcription Factors
Genes
biological evolution
ribosomes
cdc Genes
sequencing
transit
neurons
Ribosomes
Neurons
Chemical activation
Cells
activation

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

REST regulation of gene networks in adult neural stem cells. / Mukherjee, Shradha; Brulet, Rebecca; Zhang, Ling; Hsieh, Jenny.

In: Nature Communications, Vol. 7, 13360, 07.11.2016.

Research output: Contribution to journalArticle

Mukherjee, Shradha ; Brulet, Rebecca ; Zhang, Ling ; Hsieh, Jenny. / REST regulation of gene networks in adult neural stem cells. In: Nature Communications. 2016 ; Vol. 7.
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