Restoration of coupling factor activity to Escherichia coli ATPase missing the delta subunit

Jeffrey B. Smith, Paul C. Sternweis

Research output: Contribution to journalArticle

32 Scopus citations


We separated the two minor subunits (δ and ε) of the E. coli ATPase from the major subunits (α, β, and γ). The minor subunit fraction was obtained by treating purified ATPase with pyridine following the procedure that Nelson et al. (J. Biol. Chem. 348, 2049 [1973]) used to separate the subunits of chloroplast ATPase. The minor subunit fraction restored the capacity of ATPase lacking the delta subunit to recombine with ATPase-depleted membrane vesicles and to reconstitute energy coupling to the transhydrogenase and oxidative phosphorylation in the vesicles. These results clearly implicate the delta subunit in the attachment of the ATPase to the membrane.

Original languageEnglish (US)
Pages (from-to)764-771
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - Feb 3 1975


ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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