Restriction fragment length polymorphism of the gene encoding the α chain of the human T cell receptor

M. L. Hoover, J. Marks, J. Chipman, E. Palmer, P. Stastny, J. D. Capra

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Two allelic forms of the T cell antigen receptor α chain gene were discerned by restriction fragment length polymorphism (RFLP) employing the T cell antigen receptor α chain probe pGA5, and the restriction enzyme Bgl II. Analysis revealed that the polymorphic fragments are detected by a probe specific for the constant region exon of the T cell antigen receptor α chain gene. Furthermore, the polymorphic fragments were shown to segregate within families. The two allelic forms yield two homozygous states, 3.2/3.2 and 2.9/2.9, at a frequency of 76.5 and 2.9%, respectively, within the normal population. The heterozygous state was observed in 20.6% of the population. The discovery of allelic forms of both the α and β chains of the T cell antigen receptor genes may provide a unique opportunity to study heritable markers of T cell function in several human diseases.

Original languageEnglish (US)
Pages (from-to)1087-1092
Number of pages6
JournalJournal of Experimental Medicine
Volume162
Issue number3
DOIs
StatePublished - 1985

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T-Cell Antigen Receptor
Restriction Fragment Length Polymorphisms
Genes
T-Cell Receptor Genes
Population
Exons
T-Lymphocytes
Enzymes

ASJC Scopus subject areas

  • Immunology

Cite this

Restriction fragment length polymorphism of the gene encoding the α chain of the human T cell receptor. / Hoover, M. L.; Marks, J.; Chipman, J.; Palmer, E.; Stastny, P.; Capra, J. D.

In: Journal of Experimental Medicine, Vol. 162, No. 3, 1985, p. 1087-1092.

Research output: Contribution to journalArticle

Hoover, M. L. ; Marks, J. ; Chipman, J. ; Palmer, E. ; Stastny, P. ; Capra, J. D. / Restriction fragment length polymorphism of the gene encoding the α chain of the human T cell receptor. In: Journal of Experimental Medicine. 1985 ; Vol. 162, No. 3. pp. 1087-1092.
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