TY - JOUR
T1 - Restriction maps and restriction fragment length polymorphisms of the human 21-hydroxylase genes
AU - Donohoue, Patricia A.
AU - Jospe, Nicholas
AU - Migeon, Claude J.
AU - McLean, Robert H.
AU - Bias, Wilma B.
AU - White, Perrin C.
AU - Van Dop, Cornelis
N1 - Funding Information:
ACKNOWLEDGEMENTS: We thank Dr. M. C. Carroll of Harvard pK4 clone, Ms. S. F. Klupt for assistance in preparation Ms. C. Schaefer for C4 phenotyping, Drs. E. Fleischnick, Crigler, Jr., for aid in obtaining blood samples from Bw47-linked CAH, and Ms. D. Qgorzalek for preparation work was supported in part by NIH grants AM-00180 AM-31920 (R.H.M.), AM-36085 (C.V.D.) and CA-22507 University for the of restriction blots, L. Key, and J. F. two patients with HLA-of this manuscript. This (C.J.M.), AM-07116 (C.J.M.), (P.C.W.).
PY - 1986/4/29
Y1 - 1986/4/29
N2 - Restriction maps were constructed for the two human 21-hydroxylase genes (21-OHA and 21-OHB) by using DNA from subjects homozygous for a deletion of each gene. Comparing the patterns of these two genes, a KpnI restriction site occurred in the 21-OHA gene in place of a TaqI site in the 21-OHB gene about 1-kb from the 5′ end of the gene, and an extra EcoRI site was located 500 bp 5′ to the common EcoRI site. The DNA of fourteen unrelated normal subjects was digested with nine restriction endonucleases (AccI, BamHI, BglII, EcoRI, HindIII, KpnI, MspI, SacI and TaqI). Restriction fragment length polymorphisms were found with EcoRI, HindIII and AccI that resulted from polymorphic endonuclease sites outside the genes.
AB - Restriction maps were constructed for the two human 21-hydroxylase genes (21-OHA and 21-OHB) by using DNA from subjects homozygous for a deletion of each gene. Comparing the patterns of these two genes, a KpnI restriction site occurred in the 21-OHA gene in place of a TaqI site in the 21-OHB gene about 1-kb from the 5′ end of the gene, and an extra EcoRI site was located 500 bp 5′ to the common EcoRI site. The DNA of fourteen unrelated normal subjects was digested with nine restriction endonucleases (AccI, BamHI, BglII, EcoRI, HindIII, KpnI, MspI, SacI and TaqI). Restriction fragment length polymorphisms were found with EcoRI, HindIII and AccI that resulted from polymorphic endonuclease sites outside the genes.
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U2 - 10.1016/0006-291X(86)90499-7
DO - 10.1016/0006-291X(86)90499-7
M3 - Article
C2 - 3010994
AN - SCOPUS:0022448105
SN - 0006-291X
VL - 136
SP - 722
EP - 729
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -