RET rearrangements in archival oxyphilic thyroid tumors: New insights in tumorigenesis and classification of Hürthle cell carcinomas?

Petra B. Musholt, Florian Imkamp, Reinhard Von Wasielewski, Kurt W. Schmid, Thomas J. Musholt, Alan P B Dackiw, Martha A. Zeiger, Allan Siperstein

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Background. Oncocytic carcinomas (Hürthle cell carcinomas [HCCs]) are commonly considered a subgroup offollicular thyroid carcinomas (FTCs). Recent characterization of a subgroup of "Hürthle cell" papillary thyroid carcinomas (PTCs) was based on the identification of PTC-speciflc RET hybrid oncogenes in HCCs. Methods. We examined 27 HCCs, 4 oxyphilic FTCs, 5 oxyphilic PTCs, 2 poorly differentiated carcinomas arising from HCCs (HCC-UTCs), and 16 oxyphilic adenomas. Total RNA was extracted from paraffin-embedded thyroid neoplasms by a novel macrodissection technique that uses a cylindric punch. After reverse transcription-polymerase chain reaction-based screening for RET rearrangements, the samples were tested for all known RET/PTC 1 to 11 hybrids with the use of artificially constructed chimeric sequences as controls. Results. The elimination of C cells by punching dissection significantly reduced RET wild-type expression. RET hybrid oncogenes (7x RET/PTC1, 1x RET/PTC1L, 2x RET/PTC3, 5 uncharacterized RET/PTCx) were demonstrated in 7 of 27 HCCs, in 0 of 4 oxyphilic FTCs, in 4 of 5 oxyphilic PTCs, in 1 of 2 HCC-UTCs, and in 3 of 16 oxyphilic adenomas. Conclusion. Our results suggest that the expression of rearranged RET hybrid oncogenes (1) is present in a similar percentage of HCCs when compared with the literature on nonoxyphilic PTCs, (2) defines PTC-like HCCs better than histomorphologic characterization, (3) excludes HCCs as a subgroup of FTCs, and (4) may play a role in the early tumorigenesis of oncocytic tumors.

Original languageEnglish (US)
Pages (from-to)881-889
Number of pages9
JournalSurgery
Volume134
Issue number6
DOIs
StatePublished - Dec 2003

Fingerprint

Thyroid Gland
Carcinogenesis
Carcinoma
Neoplasms
Oncogenes
Oxyphilic Adenoma
Thyroid Neoplasms
Papillary Thyroid cancer
Paraffin
Reverse Transcription
Dissection
RNA
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Surgery

Cite this

RET rearrangements in archival oxyphilic thyroid tumors : New insights in tumorigenesis and classification of Hürthle cell carcinomas? / Musholt, Petra B.; Imkamp, Florian; Von Wasielewski, Reinhard; Schmid, Kurt W.; Musholt, Thomas J.; Dackiw, Alan P B; Zeiger, Martha A.; Siperstein, Allan.

In: Surgery, Vol. 134, No. 6, 12.2003, p. 881-889.

Research output: Contribution to journalArticle

Musholt, PB, Imkamp, F, Von Wasielewski, R, Schmid, KW, Musholt, TJ, Dackiw, APB, Zeiger, MA & Siperstein, A 2003, 'RET rearrangements in archival oxyphilic thyroid tumors: New insights in tumorigenesis and classification of Hürthle cell carcinomas?', Surgery, vol. 134, no. 6, pp. 881-889. https://doi.org/10.1016/j.surg.2003.08.003
Musholt, Petra B. ; Imkamp, Florian ; Von Wasielewski, Reinhard ; Schmid, Kurt W. ; Musholt, Thomas J. ; Dackiw, Alan P B ; Zeiger, Martha A. ; Siperstein, Allan. / RET rearrangements in archival oxyphilic thyroid tumors : New insights in tumorigenesis and classification of Hürthle cell carcinomas?. In: Surgery. 2003 ; Vol. 134, No. 6. pp. 881-889.
@article{694d721598a54c4593a43e24c97446d9,
title = "RET rearrangements in archival oxyphilic thyroid tumors: New insights in tumorigenesis and classification of H{\"u}rthle cell carcinomas?",
abstract = "Background. Oncocytic carcinomas (H{\"u}rthle cell carcinomas [HCCs]) are commonly considered a subgroup offollicular thyroid carcinomas (FTCs). Recent characterization of a subgroup of {"}H{\"u}rthle cell{"} papillary thyroid carcinomas (PTCs) was based on the identification of PTC-speciflc RET hybrid oncogenes in HCCs. Methods. We examined 27 HCCs, 4 oxyphilic FTCs, 5 oxyphilic PTCs, 2 poorly differentiated carcinomas arising from HCCs (HCC-UTCs), and 16 oxyphilic adenomas. Total RNA was extracted from paraffin-embedded thyroid neoplasms by a novel macrodissection technique that uses a cylindric punch. After reverse transcription-polymerase chain reaction-based screening for RET rearrangements, the samples were tested for all known RET/PTC 1 to 11 hybrids with the use of artificially constructed chimeric sequences as controls. Results. The elimination of C cells by punching dissection significantly reduced RET wild-type expression. RET hybrid oncogenes (7x RET/PTC1, 1x RET/PTC1L, 2x RET/PTC3, 5 uncharacterized RET/PTCx) were demonstrated in 7 of 27 HCCs, in 0 of 4 oxyphilic FTCs, in 4 of 5 oxyphilic PTCs, in 1 of 2 HCC-UTCs, and in 3 of 16 oxyphilic adenomas. Conclusion. Our results suggest that the expression of rearranged RET hybrid oncogenes (1) is present in a similar percentage of HCCs when compared with the literature on nonoxyphilic PTCs, (2) defines PTC-like HCCs better than histomorphologic characterization, (3) excludes HCCs as a subgroup of FTCs, and (4) may play a role in the early tumorigenesis of oncocytic tumors.",
author = "Musholt, {Petra B.} and Florian Imkamp and {Von Wasielewski}, Reinhard and Schmid, {Kurt W.} and Musholt, {Thomas J.} and Dackiw, {Alan P B} and Zeiger, {Martha A.} and Allan Siperstein",
year = "2003",
month = "12",
doi = "10.1016/j.surg.2003.08.003",
language = "English (US)",
volume = "134",
pages = "881--889",
journal = "Surgery (United States)",
issn = "0039-6060",
publisher = "Mosby Inc.",
number = "6",

}

TY - JOUR

T1 - RET rearrangements in archival oxyphilic thyroid tumors

T2 - New insights in tumorigenesis and classification of Hürthle cell carcinomas?

AU - Musholt, Petra B.

AU - Imkamp, Florian

AU - Von Wasielewski, Reinhard

AU - Schmid, Kurt W.

AU - Musholt, Thomas J.

AU - Dackiw, Alan P B

AU - Zeiger, Martha A.

AU - Siperstein, Allan

PY - 2003/12

Y1 - 2003/12

N2 - Background. Oncocytic carcinomas (Hürthle cell carcinomas [HCCs]) are commonly considered a subgroup offollicular thyroid carcinomas (FTCs). Recent characterization of a subgroup of "Hürthle cell" papillary thyroid carcinomas (PTCs) was based on the identification of PTC-speciflc RET hybrid oncogenes in HCCs. Methods. We examined 27 HCCs, 4 oxyphilic FTCs, 5 oxyphilic PTCs, 2 poorly differentiated carcinomas arising from HCCs (HCC-UTCs), and 16 oxyphilic adenomas. Total RNA was extracted from paraffin-embedded thyroid neoplasms by a novel macrodissection technique that uses a cylindric punch. After reverse transcription-polymerase chain reaction-based screening for RET rearrangements, the samples were tested for all known RET/PTC 1 to 11 hybrids with the use of artificially constructed chimeric sequences as controls. Results. The elimination of C cells by punching dissection significantly reduced RET wild-type expression. RET hybrid oncogenes (7x RET/PTC1, 1x RET/PTC1L, 2x RET/PTC3, 5 uncharacterized RET/PTCx) were demonstrated in 7 of 27 HCCs, in 0 of 4 oxyphilic FTCs, in 4 of 5 oxyphilic PTCs, in 1 of 2 HCC-UTCs, and in 3 of 16 oxyphilic adenomas. Conclusion. Our results suggest that the expression of rearranged RET hybrid oncogenes (1) is present in a similar percentage of HCCs when compared with the literature on nonoxyphilic PTCs, (2) defines PTC-like HCCs better than histomorphologic characterization, (3) excludes HCCs as a subgroup of FTCs, and (4) may play a role in the early tumorigenesis of oncocytic tumors.

AB - Background. Oncocytic carcinomas (Hürthle cell carcinomas [HCCs]) are commonly considered a subgroup offollicular thyroid carcinomas (FTCs). Recent characterization of a subgroup of "Hürthle cell" papillary thyroid carcinomas (PTCs) was based on the identification of PTC-speciflc RET hybrid oncogenes in HCCs. Methods. We examined 27 HCCs, 4 oxyphilic FTCs, 5 oxyphilic PTCs, 2 poorly differentiated carcinomas arising from HCCs (HCC-UTCs), and 16 oxyphilic adenomas. Total RNA was extracted from paraffin-embedded thyroid neoplasms by a novel macrodissection technique that uses a cylindric punch. After reverse transcription-polymerase chain reaction-based screening for RET rearrangements, the samples were tested for all known RET/PTC 1 to 11 hybrids with the use of artificially constructed chimeric sequences as controls. Results. The elimination of C cells by punching dissection significantly reduced RET wild-type expression. RET hybrid oncogenes (7x RET/PTC1, 1x RET/PTC1L, 2x RET/PTC3, 5 uncharacterized RET/PTCx) were demonstrated in 7 of 27 HCCs, in 0 of 4 oxyphilic FTCs, in 4 of 5 oxyphilic PTCs, in 1 of 2 HCC-UTCs, and in 3 of 16 oxyphilic adenomas. Conclusion. Our results suggest that the expression of rearranged RET hybrid oncogenes (1) is present in a similar percentage of HCCs when compared with the literature on nonoxyphilic PTCs, (2) defines PTC-like HCCs better than histomorphologic characterization, (3) excludes HCCs as a subgroup of FTCs, and (4) may play a role in the early tumorigenesis of oncocytic tumors.

UR - http://www.scopus.com/inward/record.url?scp=0346256746&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0346256746&partnerID=8YFLogxK

U2 - 10.1016/j.surg.2003.08.003

DO - 10.1016/j.surg.2003.08.003

M3 - Article

C2 - 14668719

AN - SCOPUS:0346256746

VL - 134

SP - 881

EP - 889

JO - Surgery (United States)

JF - Surgery (United States)

SN - 0039-6060

IS - 6

ER -