Abstract
We present evidence that retinoic acid can down-regulate transcriptional activation by the nuclear protooncogene c-jun. All three members of the retinoic acid receptor (RAR) subfamily (RARα, RARβ, and RARγ) can repress transcriptional induction of the human collagenase gene or a heterologous promoter that contains the collagenase promoter AP-1-binding site. In contrast, the retinoid X receptor fails to repress Jun/AP-1 activity, demonstrating a significant difference between the two regulatory systems through which retinoids exert their transcriptional control. Analysis of RARa mutants in transfection studies reveals that the DNA-binding domain is important for the inhibition of Jun/AP-1 activity, even though the RAR does not bind the collagenase AP-1 site. Rather, gel-retardation assays reveal that bacterially expressed full-length RARα inhibits binding of Jun protein to target DNA. These data suggest that the RARα may form a nonproductive complex with c-Jun and provides a simple mechanism by which retinoic acid may limit cell growth and possibly malignant progression.
Original language | English (US) |
---|---|
Pages (from-to) | 6092-6096 |
Number of pages | 5 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 88 |
Issue number | 14 |
DOIs | |
State | Published - Jul 15 1991 |
Keywords
- Oncogenes
- Steroid receptors
- Transcription
- Vitamin A
ASJC Scopus subject areas
- General