Retinoic acid receptors initiate induction of the cytomegalovirus enhancer in embryonal cells

Peter Ghazal, Cordell Demattei, Emi Giulietti, Steven A. Kliewer, Kazuhiko Umesono, Ronald M. Evans

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Reactivation of latent virus is believed to result from a signal transduction event that induces immediateearly (IE) gene transcription. Evidence is presented that the major IE promoter (MIEP) of human cytomegalovirus (hCMV) is activated by physiological levels of retinoic acid (RA) in human embryonal carcinoma cells. Mutagenesis experiments localized in the MIEP enhancer, a retinoic acidresponsive element composed of a direct repeat separated by five nucleotides. Protein-DNA binding experiments revealed that this element functions as a specific target site for the direct interaction of nuclear receptor proteins for RA. These findings implicate the biologically active derivative of vitamin A (RA) as a potential modulator of hCMV pathogenesis in infants and immunocompromised adults.

Original languageEnglish (US)
Pages (from-to)7630-7634
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number16
StatePublished - 1992

Fingerprint

Retinoic Acid Receptors
Tretinoin
Cytomegalovirus
Embryonal Carcinoma Stem Cells
Nucleic Acid Repetitive Sequences
DNA-Binding Proteins
Cytoplasmic and Nuclear Receptors
Nuclear Proteins
Mutagenesis
Signal Transduction
Nucleotides
Viruses
Genes

Keywords

  • Congenital defects
  • Hormone response element
  • Human cytomegalovirus
  • Pathogenesis
  • Vitamin A

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Retinoic acid receptors initiate induction of the cytomegalovirus enhancer in embryonal cells. / Ghazal, Peter; Demattei, Cordell; Giulietti, Emi; Kliewer, Steven A.; Umesono, Kazuhiko; Evans, Ronald M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 16, 1992, p. 7630-7634.

Research output: Contribution to journalArticle

Ghazal, Peter ; Demattei, Cordell ; Giulietti, Emi ; Kliewer, Steven A. ; Umesono, Kazuhiko ; Evans, Ronald M. / Retinoic acid receptors initiate induction of the cytomegalovirus enhancer in embryonal cells. In: Proceedings of the National Academy of Sciences of the United States of America. 1992 ; Vol. 89, No. 16. pp. 7630-7634.
@article{e580ffda9cc148e4a6aad6e2a80b6608,
title = "Retinoic acid receptors initiate induction of the cytomegalovirus enhancer in embryonal cells",
abstract = "Reactivation of latent virus is believed to result from a signal transduction event that induces immediateearly (IE) gene transcription. Evidence is presented that the major IE promoter (MIEP) of human cytomegalovirus (hCMV) is activated by physiological levels of retinoic acid (RA) in human embryonal carcinoma cells. Mutagenesis experiments localized in the MIEP enhancer, a retinoic acidresponsive element composed of a direct repeat separated by five nucleotides. Protein-DNA binding experiments revealed that this element functions as a specific target site for the direct interaction of nuclear receptor proteins for RA. These findings implicate the biologically active derivative of vitamin A (RA) as a potential modulator of hCMV pathogenesis in infants and immunocompromised adults.",
keywords = "Congenital defects, Hormone response element, Human cytomegalovirus, Pathogenesis, Vitamin A",
author = "Peter Ghazal and Cordell Demattei and Emi Giulietti and Kliewer, {Steven A.} and Kazuhiko Umesono and Evans, {Ronald M.}",
year = "1992",
language = "English (US)",
volume = "89",
pages = "7630--7634",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "16",

}

TY - JOUR

T1 - Retinoic acid receptors initiate induction of the cytomegalovirus enhancer in embryonal cells

AU - Ghazal, Peter

AU - Demattei, Cordell

AU - Giulietti, Emi

AU - Kliewer, Steven A.

AU - Umesono, Kazuhiko

AU - Evans, Ronald M.

PY - 1992

Y1 - 1992

N2 - Reactivation of latent virus is believed to result from a signal transduction event that induces immediateearly (IE) gene transcription. Evidence is presented that the major IE promoter (MIEP) of human cytomegalovirus (hCMV) is activated by physiological levels of retinoic acid (RA) in human embryonal carcinoma cells. Mutagenesis experiments localized in the MIEP enhancer, a retinoic acidresponsive element composed of a direct repeat separated by five nucleotides. Protein-DNA binding experiments revealed that this element functions as a specific target site for the direct interaction of nuclear receptor proteins for RA. These findings implicate the biologically active derivative of vitamin A (RA) as a potential modulator of hCMV pathogenesis in infants and immunocompromised adults.

AB - Reactivation of latent virus is believed to result from a signal transduction event that induces immediateearly (IE) gene transcription. Evidence is presented that the major IE promoter (MIEP) of human cytomegalovirus (hCMV) is activated by physiological levels of retinoic acid (RA) in human embryonal carcinoma cells. Mutagenesis experiments localized in the MIEP enhancer, a retinoic acidresponsive element composed of a direct repeat separated by five nucleotides. Protein-DNA binding experiments revealed that this element functions as a specific target site for the direct interaction of nuclear receptor proteins for RA. These findings implicate the biologically active derivative of vitamin A (RA) as a potential modulator of hCMV pathogenesis in infants and immunocompromised adults.

KW - Congenital defects

KW - Hormone response element

KW - Human cytomegalovirus

KW - Pathogenesis

KW - Vitamin A

UR - http://www.scopus.com/inward/record.url?scp=0026756101&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026756101&partnerID=8YFLogxK

M3 - Article

C2 - 1323848

AN - SCOPUS:0026756101

VL - 89

SP - 7630

EP - 7634

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 16

ER -