Retinoic acid receptors initiate induction of the cytomegalovirus enhancer in embryonal cells

Peter Ghazal; Cordell Demattei; Emi Giulietti; Steven A. Kliewer; Kazuhiko Umesono; Ronald M. Evans

doi:10.1073/pnas.89.16.7630

Retinoic acid receptors initiate induction of the cytomegalovirus enhancer in embryonal cells

Peter Ghazal, Cordell Demattei, Emi Giulietti, Steven A. Kliewer, Kazuhiko Umesono, Ronald M. Evans

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

Reactivation of latent virus is believed to result from a signal transduction event that induces immediateearly (IE) gene transcription. Evidence is presented that the major IE promoter (MIEP) of human cytomegalovirus (hCMV) is activated by physiological levels of retinoic acid (RA) in human embryonal carcinoma cells. Mutagenesis experiments localized in the MIEP enhancer, a retinoic acidresponsive element composed of a direct repeat separated by five nucleotides. Protein-DNA binding experiments revealed that this element functions as a specific target site for the direct interaction of nuclear receptor proteins for RA. These findings implicate the biologically active derivative of vitamin A (RA) as a potential modulator of hCMV pathogenesis in infants and immunocompromised adults.

Original language	English (US)
Pages (from-to)	7630-7634
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	89
Issue number	16
DOIs	https://doi.org/10.1073/pnas.89.16.7630
State	Published - 1992

Keywords

Congenital defects
Hormone response element
Human cytomegalovirus
Pathogenesis
Vitamin A

ASJC Scopus subject areas

General

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10.1073/pnas.89.16.7630

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abstract = "Reactivation of latent virus is believed to result from a signal transduction event that induces immediateearly (IE) gene transcription. Evidence is presented that the major IE promoter (MIEP) of human cytomegalovirus (hCMV) is activated by physiological levels of retinoic acid (RA) in human embryonal carcinoma cells. Mutagenesis experiments localized in the MIEP enhancer, a retinoic acidresponsive element composed of a direct repeat separated by five nucleotides. Protein-DNA binding experiments revealed that this element functions as a specific target site for the direct interaction of nuclear receptor proteins for RA. These findings implicate the biologically active derivative of vitamin A (RA) as a potential modulator of hCMV pathogenesis in infants and immunocompromised adults.",

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T1 - Retinoic acid receptors initiate induction of the cytomegalovirus enhancer in embryonal cells

AU - Ghazal, Peter

AU - Demattei, Cordell

AU - Giulietti, Emi

AU - Kliewer, Steven A.

AU - Umesono, Kazuhiko

AU - Evans, Ronald M.

PY - 1992

Y1 - 1992

N2 - Reactivation of latent virus is believed to result from a signal transduction event that induces immediateearly (IE) gene transcription. Evidence is presented that the major IE promoter (MIEP) of human cytomegalovirus (hCMV) is activated by physiological levels of retinoic acid (RA) in human embryonal carcinoma cells. Mutagenesis experiments localized in the MIEP enhancer, a retinoic acidresponsive element composed of a direct repeat separated by five nucleotides. Protein-DNA binding experiments revealed that this element functions as a specific target site for the direct interaction of nuclear receptor proteins for RA. These findings implicate the biologically active derivative of vitamin A (RA) as a potential modulator of hCMV pathogenesis in infants and immunocompromised adults.

AB - Reactivation of latent virus is believed to result from a signal transduction event that induces immediateearly (IE) gene transcription. Evidence is presented that the major IE promoter (MIEP) of human cytomegalovirus (hCMV) is activated by physiological levels of retinoic acid (RA) in human embryonal carcinoma cells. Mutagenesis experiments localized in the MIEP enhancer, a retinoic acidresponsive element composed of a direct repeat separated by five nucleotides. Protein-DNA binding experiments revealed that this element functions as a specific target site for the direct interaction of nuclear receptor proteins for RA. These findings implicate the biologically active derivative of vitamin A (RA) as a potential modulator of hCMV pathogenesis in infants and immunocompromised adults.

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KW - Hormone response element

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KW - Pathogenesis

KW - Vitamin A

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Retinoic acid receptors initiate induction of the cytomegalovirus enhancer in embryonal cells

Abstract

Keywords

ASJC Scopus subject areas

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