Retrieval of morphine-associated context induces cFos in dentate gyrus neurons

Phillip D. Rivera, Ramya K. Raghavan, Sanghee Yun, Sarah E. Latchney, Mary Katherin Mcgovern, Emily F. García, Shari G. Birnbaum, Amelia J. Eisch

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Addiction has been proposed to emerge from associations between the drug and the reward-associated contexts. This associative learning has a cellular correlate, as there are more cFos+ neurons in the hippocampal dentate gyrus (DG) after psychostimulant conditioned place preference (CPP) versus saline controls. However, it is unknown whether morphine CPP leads to a similar DG activation, or whether DG activation is due to locomotion, handling, pharmacological effects, or-as data from contextual fear learning suggests-exposure to the drug-associated context. To explore this, we employed an unbiased, counterbalanced, and shortened CPP design that led to place preference and more DG cFos+ cells. Next, mice underwent morphine CPP but were then sequestered into the morphine-paired (conditioned stimulus+ [CS+]) or saline-paired (CS-) context on test day. Morphine-paired mice sequestered to CS+ had ∼30% more DG cFos+ cells than saline-paired mice. Furthermore, Bregma analysis revealed morphine-paired mice had more cFos+ cells in CS+ compared to CS- controls. Notably, there was no significant difference in DG cFos+ cell number after handling alone or after receiving morphine in home cage. Thus, retrieval of morphine-associated context is accompanied by activation of hippocampal DG granule cell neurons.

Original languageEnglish (US)
Pages (from-to)409-414
Number of pages6
JournalHippocampus
Volume25
Issue number4
DOIs
StatePublished - Apr 1 2015

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Dentate Gyrus
Morphine
Neurons
Parahippocampal Gyrus
Learning
Locomotion
Reward
Pharmaceutical Preparations
Fear
Cell Count
Pharmacology

Keywords

  • Addiction
  • Conditioned place preference
  • Hippocampus
  • Immediate early gene
  • Re-exposure

ASJC Scopus subject areas

  • Cognitive Neuroscience

Cite this

Rivera, P. D., Raghavan, R. K., Yun, S., Latchney, S. E., Mcgovern, M. K., García, E. F., ... Eisch, A. J. (2015). Retrieval of morphine-associated context induces cFos in dentate gyrus neurons. Hippocampus, 25(4), 409-414. https://doi.org/10.1002/hipo.22393

Retrieval of morphine-associated context induces cFos in dentate gyrus neurons. / Rivera, Phillip D.; Raghavan, Ramya K.; Yun, Sanghee; Latchney, Sarah E.; Mcgovern, Mary Katherin; García, Emily F.; Birnbaum, Shari G.; Eisch, Amelia J.

In: Hippocampus, Vol. 25, No. 4, 01.04.2015, p. 409-414.

Research output: Contribution to journalArticle

Rivera, PD, Raghavan, RK, Yun, S, Latchney, SE, Mcgovern, MK, García, EF, Birnbaum, SG & Eisch, AJ 2015, 'Retrieval of morphine-associated context induces cFos in dentate gyrus neurons', Hippocampus, vol. 25, no. 4, pp. 409-414. https://doi.org/10.1002/hipo.22393
Rivera PD, Raghavan RK, Yun S, Latchney SE, Mcgovern MK, García EF et al. Retrieval of morphine-associated context induces cFos in dentate gyrus neurons. Hippocampus. 2015 Apr 1;25(4):409-414. https://doi.org/10.1002/hipo.22393
Rivera, Phillip D. ; Raghavan, Ramya K. ; Yun, Sanghee ; Latchney, Sarah E. ; Mcgovern, Mary Katherin ; García, Emily F. ; Birnbaum, Shari G. ; Eisch, Amelia J. / Retrieval of morphine-associated context induces cFos in dentate gyrus neurons. In: Hippocampus. 2015 ; Vol. 25, No. 4. pp. 409-414.
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