REV-ERBα participates in circadian SREBP signaling and bile acid homeostasis

Gwendal Le Martelot, Thierry Claudel, David Gatfield, Olivier Schaad, Benoît Kornmann, Giuseppe Lo Sasso, Antonio Moschetta, Ueli Schibler

Research output: Contribution to journalArticle

241 Citations (Scopus)

Abstract

In mammals, many aspects of behavior and physiology, and in particular cellular metabolism, are coordinated by the circadian timing system. Molecular clocks are thought to rely on negative feedback loops in clock gene expression that engender oscillations in the accumulation of transcriptional regulatory proteins, such as the orphan receptor REV-ERBα. Circadian transcription factors then drive daily rhythms in the expression of clock-controlled output genes, for example genes encoding enzymes and regulators of cellular metabolism. To gain insight into clock output functions of REV-ERBα, we carried out genome-wide transcriptome profiling experiments with liver RNA from wild-type mice, Rev-erbα knock-out mice, or REV-ERBα overexpressing mice. On the basis of these genetic loss- and gain-of-function experiments, we concluded that REV-ERBα participates in the circadian modulation of sterol regulatory element-binding protein (SREBP) activity, and thereby in the daily expression of SREBP target genes involved in cholesterol and lipid metabolism. This control is exerted via the cyclic transcription of Insig2, encoding a trans-membrane protein that sequesters SREBP proteins to the endoplasmic reticulum membranes and thereby interferes with the proteolytic activation of SREBPs in Golgi membranes. REV-ERBα also participates in the cyclic expression of cholesterol-7α-hydroxylase (CYP7A1), the rate-limiting enzyme in converting cholesterol to bile acids. Our findings suggest that this control acts via the stimulation of LXR nuclear receptors by cyclically produced oxysterols. In conclusion, our study suggests that rhythmic cholesterol and bile acid metabolism is not just driven by alternating feeding-fasting cycles, but also by REV-ERBα, a component of the circadian clockwork circuitry.

Original languageEnglish (US)
Article numbere1000181
JournalPLoS Biology
Volume7
Issue number9
DOIs
StatePublished - 2009

Fingerprint

Sterol Regulatory Element Binding Proteins
bile acids
Bile Acids and Salts
sterols
binding proteins
Clocks
homeostasis
Homeostasis
Cholesterol
cholesterol
Metabolism
metabolism
Genes
mice
Cholesterol 7-alpha-Hydroxylase
cholesterol metabolism
receptors
Circadian Clocks
transmembrane proteins
Membranes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Le Martelot, G., Claudel, T., Gatfield, D., Schaad, O., Kornmann, B., Lo Sasso, G., ... Schibler, U. (2009). REV-ERBα participates in circadian SREBP signaling and bile acid homeostasis. PLoS Biology, 7(9), [e1000181]. https://doi.org/10.1371/journal.pbio.1000181

REV-ERBα participates in circadian SREBP signaling and bile acid homeostasis. / Le Martelot, Gwendal; Claudel, Thierry; Gatfield, David; Schaad, Olivier; Kornmann, Benoît; Lo Sasso, Giuseppe; Moschetta, Antonio; Schibler, Ueli.

In: PLoS Biology, Vol. 7, No. 9, e1000181, 2009.

Research output: Contribution to journalArticle

Le Martelot, G, Claudel, T, Gatfield, D, Schaad, O, Kornmann, B, Lo Sasso, G, Moschetta, A & Schibler, U 2009, 'REV-ERBα participates in circadian SREBP signaling and bile acid homeostasis', PLoS Biology, vol. 7, no. 9, e1000181. https://doi.org/10.1371/journal.pbio.1000181
Le Martelot G, Claudel T, Gatfield D, Schaad O, Kornmann B, Lo Sasso G et al. REV-ERBα participates in circadian SREBP signaling and bile acid homeostasis. PLoS Biology. 2009;7(9). e1000181. https://doi.org/10.1371/journal.pbio.1000181
Le Martelot, Gwendal ; Claudel, Thierry ; Gatfield, David ; Schaad, Olivier ; Kornmann, Benoît ; Lo Sasso, Giuseppe ; Moschetta, Antonio ; Schibler, Ueli. / REV-ERBα participates in circadian SREBP signaling and bile acid homeostasis. In: PLoS Biology. 2009 ; Vol. 7, No. 9.
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