Reversal of cigarette smoke extract-induced sinonasal epithelial cell barrier dysfunction through Nrf2 Activation

Anuj Tharakan, Ashleigh A. Halderman, Andrew P. Lane, Shyam Biswal, Murugappan Ramanathan

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: Environmental factors such as inhaled pollutants like cigarette smoke may play a significant role in diseases of the upper airway including chronic rhinosinusitis (CRS). Recent studies have shown that cigarette smoke causes impaired airway epithelial cell barrier function likely through environmental oxidative stress related pathways. The purpose of this study is to explore whether enhancing nuclear factor erythroid 2 [NF-E2]-related factor 2 [Nrf2], the body's master antioxidant system, can ameliorate cigarette smoke–induced sinonasal epithelial cell (SNEC) barrier dysfunction. Methods: Human SNECs (HSNECs) were grown from control patients at the air-liquid interface (ALI). HSNECs were stimulated with cigarette smoke extract (CSE) with and without pharmacologic activation of Nrf2. HSNECs were then stained for the epithelial cell junctional proteins zonula occludens 1 (ZO-1) and junctional adhesion molecule A (JAM-A) using confocal microscopy. In addition, transepithelial electrical resistance (TER) was measured in cultures before and after stimulation with CSE. Results: CSE stimulation caused a global disruption of the epithelial junctional proteins ZO-1 and JAM-A along with an associated decrease in TER levels. Enhancing Nrf2 levels prior to stimulation with CSE was associated with increased localization of ZO-1 and JAM-A levels at the cell surface and statistically significant increases in TER levels. Conclusion: This is the first study to demonstrate that cigarette smoke induced SNEC barrier dysfunction is reversible by Nrf2 activation. The Nrf2 antioxidant pathway may represent a potential therapeutic target for cigarette smoke–associated sinonasal inflammation.

Original languageEnglish (US)
Pages (from-to)1145-1150
Number of pages6
JournalInternational Forum of Allergy and Rhinology
Volume6
Issue number11
DOIs
StatePublished - Nov 1 2016

Fingerprint

NF-E2-Related Factor 2
Smoke
Tobacco Products
Epithelial Cells
Junctional Adhesion Molecule A
Electric Impedance
Zonula Occludens-1 Protein
Antioxidants
Tight Junctions
Confocal Microscopy
Oxidative Stress
Air
Inflammation

Keywords

  • chronic rhinosinusitis
  • cigarette smoke
  • epithelial barrier
  • Nrf2
  • sinonasal epithelium
  • tight junctions

ASJC Scopus subject areas

  • Immunology and Allergy
  • Otorhinolaryngology

Cite this

Reversal of cigarette smoke extract-induced sinonasal epithelial cell barrier dysfunction through Nrf2 Activation. / Tharakan, Anuj; Halderman, Ashleigh A.; Lane, Andrew P.; Biswal, Shyam; Ramanathan, Murugappan.

In: International Forum of Allergy and Rhinology, Vol. 6, No. 11, 01.11.2016, p. 1145-1150.

Research output: Contribution to journalArticle

Tharakan, Anuj ; Halderman, Ashleigh A. ; Lane, Andrew P. ; Biswal, Shyam ; Ramanathan, Murugappan. / Reversal of cigarette smoke extract-induced sinonasal epithelial cell barrier dysfunction through Nrf2 Activation. In: International Forum of Allergy and Rhinology. 2016 ; Vol. 6, No. 11. pp. 1145-1150.
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