RGS proteins provide biochemical control of agonist-evoked [Ca2+]i oscillations

Xiang Luo, Serguei Popov, Amal Kanti Bera, Thomas M. Wilkie, Shmuel Muallem

Research output: Contribution to journalArticle

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Abstract

Agonist-evoked [Ca2+]i oscillations have been considered a biophysical phenomenon reflecting the regulation of the IP3 receptor by [Ca2+]i. Here we show that [Ca2+]i oscillations are a biochemical phenomenon emanating from regulation of Ca2+ signaling by the regulators of G protein signaling (RGS) proteins. [Ca2+]i oscillations evoked by G protein-coupled receptors require the action of RGS proteins. Inhibition of endogenous RGS protein action disrupted agonist-evoked [Ca2+]i oscillations by a stepwise conversion to a sustained response. Based on these findings and the effect of mutant RGS proteins and anti-RGS protein antibodies on Ca2+ signaling, we propose that RGS proteins within the G protein-coupled receptor complexes provide a biochemical control of [Ca2+]i oscillations.

Original languageEnglish (US)
Pages (from-to)651-660
Number of pages10
JournalMolecular Cell
Volume7
Issue number3
DOIs
StatePublished - Mar 1 2001

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RGS Proteins
G-Protein-Coupled Receptors
Biophysical Phenomena
Biochemical Phenomena
Inositol 1,4,5-Trisphosphate Receptors
Antibodies

ASJC Scopus subject areas

  • Molecular Biology

Cite this

RGS proteins provide biochemical control of agonist-evoked [Ca2+]i oscillations. / Luo, Xiang; Popov, Serguei; Bera, Amal Kanti; Wilkie, Thomas M.; Muallem, Shmuel.

In: Molecular Cell, Vol. 7, No. 3, 01.03.2001, p. 651-660.

Research output: Contribution to journalArticle

Luo, Xiang ; Popov, Serguei ; Bera, Amal Kanti ; Wilkie, Thomas M. ; Muallem, Shmuel. / RGS proteins provide biochemical control of agonist-evoked [Ca2+]i oscillations. In: Molecular Cell. 2001 ; Vol. 7, No. 3. pp. 651-660.
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