Rhizobial Nod factors stimulate somatic embryo development in Picea abies

J. V. Dyachok, A. E. Tobin, N. P J Price, S. Von Arnold

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Nod factors are lipochitooligosaccharides (LCOs) secreted by rhizobia. Nod factors trigger the nodulation programme in a compatible host. A bioassay was set up to test how crude (NGR234) and purified (NodS) Nod factors influence cell division and somatic embryogenesis in a conifer, Norway spruce (Picea abies). The Nod factors promoted cell division in the absence of auxin and cytokinin. More detailed studies showed that Nods stimulates development of proembryogenic masses from small cell aggregates and further embryo development. However, stimulation was only observed in low-density cell cultures. Our data suggest that rhizobial Nod factors substitute for conditioning factors in embryogenic cultures of Norway spruce.

Original languageEnglish (US)
Pages (from-to)290-297
Number of pages8
JournalPlant Cell Reports
Volume19
Issue number3
StatePublished - Jan 2000

Fingerprint

somatic embryos
Picea abies
embryogenesis
cell division
cell aggregates
nodulation
somatic embryogenesis
Rhizobium
cytokinins
conifers
auxins
cell culture
bioassays
testing

Keywords

  • Embryo development
  • Nod factors
  • Picea abies
  • Protoplast division
  • Somatic embryogenesis

ASJC Scopus subject areas

  • Plant Science

Cite this

Dyachok, J. V., Tobin, A. E., Price, N. P. J., & Von Arnold, S. (2000). Rhizobial Nod factors stimulate somatic embryo development in Picea abies. Plant Cell Reports, 19(3), 290-297.

Rhizobial Nod factors stimulate somatic embryo development in Picea abies. / Dyachok, J. V.; Tobin, A. E.; Price, N. P J; Von Arnold, S.

In: Plant Cell Reports, Vol. 19, No. 3, 01.2000, p. 290-297.

Research output: Contribution to journalArticle

Dyachok, JV, Tobin, AE, Price, NPJ & Von Arnold, S 2000, 'Rhizobial Nod factors stimulate somatic embryo development in Picea abies', Plant Cell Reports, vol. 19, no. 3, pp. 290-297.
Dyachok JV, Tobin AE, Price NPJ, Von Arnold S. Rhizobial Nod factors stimulate somatic embryo development in Picea abies. Plant Cell Reports. 2000 Jan;19(3):290-297.
Dyachok, J. V. ; Tobin, A. E. ; Price, N. P J ; Von Arnold, S. / Rhizobial Nod factors stimulate somatic embryo development in Picea abies. In: Plant Cell Reports. 2000 ; Vol. 19, No. 3. pp. 290-297.
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