Rhizobial Nod factors stimulate somatic embryo development in Picea abies

J. V. Dyachok, A. E. Tobin, N. P J Price, S. Von Arnold

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

Nod factors are lipochitooligosaccharides (LCOs) secreted by rhizobia. Nod factors trigger the nodulation programme in a compatible host. A bioassay was set up to test how crude (NGR234) and purified (NodS) Nod factors influence cell division and somatic embryogenesis in a conifer, Norway spruce (Picea abies). The Nod factors promoted cell division in the absence of auxin and cytokinin. More detailed studies showed that Nods stimulates development of proembryogenic masses from small cell aggregates and further embryo development. However, stimulation was only observed in low-density cell cultures. Our data suggest that rhizobial Nod factors substitute for conditioning factors in embryogenic cultures of Norway spruce.

Original languageEnglish (US)
Pages (from-to)290-297
Number of pages8
JournalPlant Cell Reports
Volume19
Issue number3
DOIs
StatePublished - Jan 2000

Keywords

  • Embryo development
  • Nod factors
  • Picea abies
  • Protoplast division
  • Somatic embryogenesis

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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    Dyachok, J. V., Tobin, A. E., Price, N. P. J., & Von Arnold, S. (2000). Rhizobial Nod factors stimulate somatic embryo development in Picea abies. Plant Cell Reports, 19(3), 290-297. https://doi.org/10.1007/s002990050015