RhoA required for acid-induced stress fiber formation and trafficking and activation of NHE3

Xiaojing Yang, Hai Chang Huang, Helen Yin, Robert J. Alpern, Patricia A. Preisig

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Exposure to an acid load increases apical membrane Na+/H + antiporter (NHE3) activity, a process that involves exocytic trafficking of the transporter to the apical membrane. We have previously shown that an intact microfilament structure is required for this exocytic process (Yang X, Amemiya M, Peng Y, Moe OW, Preisig PA, Alpern RJ. Am J Physiol Cell Physiol 279: C410-C419, 2000). The present studies demonstrate that acid-induced stress fiber formation is required for stimulation of NHE3 activity. Formation of stress fibers is associated with acid-induced tyrosine phosphorylation and increases in protein abundance of two focal adhesion proteins, p125 FAK and paxillin. The Rho kinase inhibitor Y27632 completely blocks acid-induced stress fiber formation and the increases in apical membrane NHE3 abundance and activity, but it has no effect on acid-induced tyrosine phosphorylation of p125FAK or paxillin. Herbimycin A completely blocks acid-induced tyrosine phosphorylation of p125FAK and paxillin but only partially blocks stress fiber formation and NHE3 activation. These studies demonstrate that Rho kinase mediates acid-induced stress fiber formation, which is required for NHE3 exocytosis, and increases in NHE3 activity. Acid-induced tyrosine phosphorylation of the focal adhesion proteins p125FAK and paxillin is not Rho kinase dependent. Thus these two acid-mediated effects are associated, yet independent processes.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume293
Issue number4
DOIs
StatePublished - Oct 2007

Fingerprint

Stress Fibers
Paxillin
Acids
rho-Associated Kinases
Tyrosine
Phosphorylation
Focal Adhesions
Focal Adhesion Kinase 1
Membranes
Sodium-Hydrogen Antiporter
Exocytosis
Actin Cytoskeleton
Proteins

Keywords

  • Actin
  • OKP cells
  • Tyrosine phosphorylation
  • Y27632

ASJC Scopus subject areas

  • Physiology

Cite this

RhoA required for acid-induced stress fiber formation and trafficking and activation of NHE3. / Yang, Xiaojing; Huang, Hai Chang; Yin, Helen; Alpern, Robert J.; Preisig, Patricia A.

In: American Journal of Physiology - Renal Physiology, Vol. 293, No. 4, 10.2007.

Research output: Contribution to journalArticle

Yang, Xiaojing ; Huang, Hai Chang ; Yin, Helen ; Alpern, Robert J. ; Preisig, Patricia A. / RhoA required for acid-induced stress fiber formation and trafficking and activation of NHE3. In: American Journal of Physiology - Renal Physiology. 2007 ; Vol. 293, No. 4.
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