Ribonucleoprotein structure in nascent hnRNA is nonrandom and sequence-dependent

Ann L. Beyer, Oscar L. Miller, Steven L. McKnight

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

We have studied the ribonucleoprotein morphology of nascent hnRNA using chromatin spreading methods. This approach allows visualization of multiple transcripts of the same DNA sequence. Thus the RNP structure of hnRNA molecules with the same nucleotide sequence can be compared. We find that RNP particles averaging 240 Å in diameter occur on the majority of hnRNA transcription units, but that RNA fibrils are generally not completely covered with particles. Furthermore, the RNP particle location is nonrandom for the transcripts of a given gene. Particle number and arrangement on RNP fibrils vary from one transcription unit to the next, and are not obviously related to transcription unit size or activity. Analysis of particle location with respect to the 5′ RNA terminus allows RNP particle localization to homologous sequences of less than 500 ribonucleotides on the different fibrils of a single transcription unit. RNP particles assemble very soon after synthesis of the particular sequence. Sites of particle formation are distinct from sites of double-stranded hairpin loops on the RNA fibrils. A differential, or differentially stable, RNP structure with respect to RNA sequence may bear on mechanisms of specific hnRNA processing.

Original languageEnglish (US)
Pages (from-to)75-84
Number of pages10
JournalCell
Volume20
Issue number1
DOIs
StatePublished - 1980

Fingerprint

Ribonucleoproteins
Transcription
RNA
Ribonucleotides
Sequence Homology
Chromatin
DNA sequences
Visualization
Nucleotides
Genes
Molecules
Processing

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Ribonucleoprotein structure in nascent hnRNA is nonrandom and sequence-dependent. / Beyer, Ann L.; Miller, Oscar L.; McKnight, Steven L.

In: Cell, Vol. 20, No. 1, 1980, p. 75-84.

Research output: Contribution to journalArticle

Beyer, Ann L. ; Miller, Oscar L. ; McKnight, Steven L. / Ribonucleoprotein structure in nascent hnRNA is nonrandom and sequence-dependent. In: Cell. 1980 ; Vol. 20, No. 1. pp. 75-84.
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